CRISPR/Cas9-engineered mutation to identify the roles of phytochromes in regulating photomorphogenesis and flowering time in soybean
CRISPR/Cas9-engineered mutation to identify the roles of phytochromes in regulating photomorphogenesis and flowering time in soybean
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CRISPR/Cas9 工程突变鉴定光敏色素在调节大豆光形态建成和开花时间中的作用
DOI：
10.1016/j.cj.2022.03.008
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发表时间：
2022-04
期刊：
影响因子：
--
通讯作者：
Yanxi Pei
中科院分区：
文献类型：
--
作者：
Fen Zhao;Xiangguang Lyu;Ronghuan Ji;Jun Liu;Tao Zhao;Hongyu Li;Bin Liu;Yanxi Pei
大豆（Glycine max）对环境光变化的响应是通过多种形态的改变，从而影响其在田间的产量潜力和稳定性。光敏色素（phyys）是植物特异性的红光(R)和远红光（FR）光感受器，介导光形态发生和光周期开花。作为一种古老的四倍体，大豆含有4个phya、2个ophyb和2个ophye近亲。除了GmPHYA2/E4和GmPHYA3/E3已被确定为光周期依赖性开花抑制因子外，gmphyys的功能仍不清楚。我们使用CRISPR/Cas9技术生成了一系列针对gmphyaorgmphybgenes的单个或组合突变。表型分析表明，GmPHYB1主要介导r光诱导的光形态发生，而GmPHYA2/E4和GmPHYA3/E3，其次是GmPHYA1和GmPHYB2，在幼苗期介导FR光响应中起冗余和加性作用。在自然长日照条件下，GmPHYA2/E4和GmPHYA3/E3的花期延迟，受GmPHYA1和GmPHYA4的影响较弱。本研究证实了GmPHYAs和gmphyb在调节光响应中的多种功能，并为鉴定其调控大豆农艺性状的功能机制提供了一组核心光敏色素突变等位基因。
Soybean (Glycine max) responds to ambient light variation by undergoing multiform morphological alterations, influencing its yield potential and stability in the field. Phytochromes (PHYs) are plant-specific red (R) and far-red (FR) light photoreceptors mediating photomorphogenesis and photoperiodic flowering. As an ancient tetraploid, soybean harbors fourPHYA, twoPHYB, and twoPHYEparalogs. Except for GmPHYA2/E4 and GmPHYA3/E3, which have been identified as photoperiod-dependent flowering repressors, the functions of GmPHYs are still largely unclear. We generated a series of individual or combined mutations targeting theGmPHYAorGmPHYBgenes using CRISPR/Cas9 technology. Phenotypic analysis revealed that GmPHYB1 mediates predominantly R-light induced photomorphogenesis, whereas GmPHYA2/E4 and GmPHYA3/E3, followed by GmPHYA1 and GmPHYB2, function redundantly and additively in mediating FR light responses in seedling stage. GmPHYA2/E4 and GmPHYA3/E3, with weak influence from GmPHYA1 and GmPHYA4, delay flowering time under natural long-day conditions. This study has demonstrated the diversified functions of GmPHYAs and GmPHYBs in regulating light response, and provides a core set of phytochrome mutant alleles for characterization of their functional mechanisms in regulating agronomic traits of soybean.
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影响因子：
11.6
作者：
Takano, M;Inagaki, N;Shinomura, T
通讯作者：
Shinomura, T
DOI：
10.1073/pnas.0910446107
发表时间：
2010-03
期刊：
Proceedings of the National Academy of Sciences
影响因子：
--
作者：
B. Strasser;M. Sánchez-Lamas;M. Yanovsky;J. Casal;P. Cerdán
通讯作者：
B. Strasser;M. Sánchez-Lamas;M. Yanovsky;J. Casal;P. Cerdán
影响因子：
64.5
作者：
Pedmale UV;Huang SC;Zander M;Cole BJ;Hetzel J;Ljung K;Reis PAB;Sridevi P;Nito K;Nery JR;Ecker JR;Chory J
通讯作者：
Chory J
影响因子：
14.9
作者：
Grant D;Nelson RT;Cannon SB;Shoemaker RC
通讯作者：
Shoemaker RC
影响因子：
2.8
作者：
T. Shinomura
通讯作者：
T. Shinomura