Proteomic analysis of S-acylated proteins in human B cells reveals palmitoylation of the immune regulators CD20 and CD23.
Proteomic analysis of S-acylated proteins in human B cells reveals palmitoylation of the immune regulators CD20 and CD23.
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DOI：
10.1371/journal.pone.0037187
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Journet A
中科院分区：
文献类型：
--
作者：
Ivaldi C;Martin BR;Kieffer-Jaquinod S;Chapel A;Levade T;Garin J;Journet A
S-棕榈酰化是一种可逆的翻译后修饰，对控制多种膜蛋白的膜靶向性和功能具有重要意义，这些膜蛋白在信号转导、支架和运输中具有不同的作用。我们试图利用酰基-生物素交换化学，结合液相色谱-串联质谱仪的差示分析，在B淋巴细胞中鉴定新的棕榈酰化蛋白。总而言之，我们从人EBV转化的淋巴样细胞中鉴定出57个新的棕榈酰化蛋白候选。其中CD20和CD23(低亲和力免疫球蛋白epsilon Fc受体)是免疫调节剂，是治疗血液系统恶性肿瘤、自身免疫性疾病和变态反应性疾病的有效/潜在的治疗靶点。丙氨酸突变体的异源表达结合生物正交代谢标记证实了CD20和CD23的棕榈酰化。这项研究展示了B细胞中一组新的棕榈酰化蛋白，说明了蛋白棕榈酰化在免疫调节中的普遍作用。
S-palmitoylation is a reversible post-translational modification important for controlling the membrane targeting and function of numerous membrane proteins with diverse roles in signalling, scaffolding, and trafficking. We sought to identify novel palmitoylated proteins in B lymphocytes using acyl-biotin exchange chemistry, coupled with differential analysis by liquid-chromatography tandem mass spectrometry. In total, we identified 57 novel palmitoylated protein candidates from human EBV-transformed lymphoid cells. Two of them, namely CD20 and CD23 (low affinity immunoglobulin epsilon Fc receptor), are immune regulators that are effective/potential therapeutic targets for haematological malignancies, autoimmune diseases and allergic disorders. Palmitoylation of CD20 and CD23 was confirmed by heterologous expression of alanine mutants coupled with bioorthogonal metabolic labeling. This study demonstrates a new subset of palmitoylated proteins in B cells, illustrating the ubiquitous role of protein palmitoylation in immune regulation.
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