The steroid hormone 20-hydroxyecdysone upregulated the protein phosphatase 6 for the programmed cell death in the insect midgut
The steroid hormone 20-hydroxyecdysone upregulated the protein phosphatase 6 for the programmed cell death in the insect midgut
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类固醇激素 20-羟基蜕皮酮上调蛋白质磷酸酶 6，从而导致昆虫中肠的程序性细胞死亡
DOI：
10.1007/s00726-011-1159-9
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发表时间：
2012-08
期刊：
影响因子：
3.5
通讯作者：
Xiao-Fan Zhao
中科院分区：
文献类型：
--
作者：
Chuan-Xu Wang;Wei-Wei Zheng;Peng-Cheng Liu;Jin-Xing Wang;Xiao-Fan Zhao
细胞程序性死亡（PCD）在昆虫变态过程中中肠的重塑中起着重要作用。昆虫中肠PCD由类固醇激素20-羟基蜕皮激素（20 E）触发，并由一系列基因介导。然而，20 E触发中肠PCD的机制仍不清楚。本文报道了一种蛋白磷酸酶6（PP 6）在棉铃虫中肠PCD中的作用，PP 6在幼虫生长过程中在中肠表达，在变态过程中表达量显著增加。这种增加被证明是由20 E调节的。保幼激素类似物methoprene对PP 6的表达没有影响。RNA干扰分析表明，20 E通过蜕皮激素受体EcRB 1上调PP 6的转录水平，通过幼虫取食或注射PP 6 dsRNA抑制PP 6的表达，导致变态期中肠PCD的抑制。其机制可能是通过抑制20 E信号通路或中肠PCD相关基因Broad（Br）、E74 a、E75 b、HR 3、E93、rpr和caspase等。这些结果表明，PP 6参与了20 E信号转导途径，并参与了中肠的PCD。
Programmed cell death (PCD) plays an important role in insect midgut remodeling during metamorphosis. Insect midgut PCD is triggered by the steroid hormone 20-hydroxyecdysone (20E) and it is mediated by a series of genes. However, the mechanism by which 20E triggers midgut PCD is still unclear. Here, we report a protein phosphatase 6 (PP6) fromHelicoverpa armigeraplaying roles in midgut PCD.PP6was expressed in the midgut during larval growth and it is significantly increased during metamorphosis. The increase was proven to be regulated by 20E. The juvenile hormone analog methoprene has no effect onPP6expression. RNA interference analysis suggests that 20E upregulated thePP6transcript levels through the ecdysone receptorEcRB1.PP6knockdown by larval feeding or PP6 dsRNA injection resulted in the repression of the midgut PCD during the metamorphic stage. The mechanism was demonstrated to be through the suppression of genes such asBroad (Br),E74a, E75b, HR3, E93, rpr, andcaspase, which are involved in 20E signaling pathway or midgut PCD. These findings suggest that PP6 is involved in the 20E signal transduction pathway and participates in the PCD in midgut.
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影响因子：
4.6
作者：
Zhang Wei;Youping Yin;Basheng Zhang;Zhong-kang Wang;Guoxiong Peng;Yueqing Cao;Yuxian Xia
通讯作者：
Zhang Wei;Youping Yin;Basheng Zhang;Zhong-kang Wang;Guoxiong Peng;Yueqing Cao;Yuxian Xia
影响因子：
3.8
作者：
Fabrick, JA;Baker, JE;Kanost, MR
通讯作者：
Kanost, MR
影响因子：
3.8
作者：
Nishiura, JT;Ray, K;Murray, J
通讯作者：
Murray, J
DOI：
--
发表时间：
1991
期刊：
Advances in second messenger and phosphoprotein research
影响因子：
--
作者：
S. Shenolikar;A. Nairn
通讯作者：
S. Shenolikar;A. Nairn
DOI：
10.1111/j.1432-1033.1995.0533k.x
发表时间：
1995-04-15
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
KUHELJ, R;DOLINAR, M;TURK, V
通讯作者：
TURK, V