Axonal Misdirection as Contributing Factor to Aberrant Reinnervation of Muscles After Facial Nerve Suture in Cats
Axonal Misdirection as Contributing Factor to Aberrant Reinnervation of Muscles After Facial Nerve Suture in Cats
复制标题
轴突误导是猫面神经缝合后肌肉神经支配异常的影响因素
DOI：
10.1076/apab.111.3.273.23460
复制
发表时间：
2003
影响因子：
3
通讯作者：
R. Romansky
中科院分区：
文献类型：
--
作者：
R. Romansky
而轴突切断后肌肉神经再支配的基本特征已被广泛研究，在大鼠中，很少有人知道猫的轴突再生和寻路。为了解决这个问题，成年猫进行了面部-面部吻合术（FFA）。第一组用于建立用于将1，1 ′-二异丙基-3，3，3，3 ′-四叔胺甲基酞菁高氯酸盐（DiI）和固蓝（FB）放置在相应的横断神经上来标记颅颌面支和颊面支的最佳参数。第二组动物在右侧面神经切断和缝合后3个月进行相同的双侧标记。这一组用来确定运动神经元的数量，这些神经元在手术后分支并投射到两侧面神经分支。在对照侧，DiI应用于颧眶分支标记为3883 ± 598（平均值± S.D.）面神经背侧亚核和面神经中间亚核的胞体数为1617 ± 552个，而颊分支的胞体数为1617 ± 552个。没有双标记细胞。FFA后3个月，逆行标记的运动神经元散在分布于整个面神经核。为了确定胞体的比例，即多个轴突分支重新生长到两个神经中，从数字图像中计数双标记（FB + DiI）运动神经元。颧眶神经含有3311 ± 430个DiI标记的运动神经元，颊神经含有1500 ± 442个FB标记的运动神经元。双标记胞体（DiI+FB）的出现数为311 ± 103，表明大约6%的逆行标记运动神经元将轴突分支到两条神经中。我的结论是，故障轴突寻路，而不是偏离神经再支配后FFA猫的功能恢复差。
Whereas basic features of post-axotomy muscle reinnervation have been extensively studied in rats, little is known about axonal regrowth and pathfinding in cats. To address the question, adult cats were subjected to facial-facial anastomosis (FFA). First group served to establish optimal parameters for labeling of the zygomatic and buccal facial branches with 1, 1 ' d i o c t a d e c y l - 3, 3, 3, ' 3 ' - t e t r a m e t h y l i n d o - carbocyanine perchlorate (DiI) and Fast Blue (FB) placed onto respective transected nerves. The second group of animals underwent identical bilateral labeling 3 months after transection and suture of the right facial nerve. This group served to establish the number of motoneurons, which had branched after surgery and projected into both facial branches. On control side, DiI application onto zygomatico-orbital branch labeled 3883 ± 598 (mean ± S.D.) perikarya were confined to the dorsal and intermediate facial subnuclei, meanwhile an application of FB onto the buccal branch labeled 1617 ± 552 perikarya in the lateral and ventrolateral subnuclei. There were no double-labeled cells. Three months after FFA all retrogradely labeled motoneurons were scattered throughout the entire facial nucleus. To establish the proportion of perikarya, that re-grew multiple axonal branches into both nerves, double-labeled (FB + DiI) motoneurons were counted from digital images. The zygomatico-orbital nerve contained 3311 ± 430 DiI-labeled whereas the buccal nerve 1500 ± 442 FB-labeled motoneurons. The occurrence of 311 ± 103 double-labeled perikarya (DiI+FB) suggested that approximately 6% of all retrogradely labeled motoneurons branched axons into both nerves. I conclude that malfunctioning axonal pathfinding rather than deviant reinnervation contributed to poor recovery of function after FFA in the cat.
DOI：
10.1113/jphysiol.1981.sp013664
发表时间：
1981-04
期刊：
The Journal of Physiology
影响因子：
--
作者：
K. Horch;S. Lisney
通讯作者：
K. Horch;S. Lisney
DOI：
10.1073/pnas.85.5.1701
发表时间：
1988-03-01
影响因子：
11.1
作者：
BAICHWAL, RR;BIGBEE, JW;DEVRIES, GH
通讯作者：
DEVRIES, GH
神经营养素对体内哺乳动物运动神经元的影响。
DOI：
10.1002/neu.480241202
发表时间：
1993
期刊：
Journal of neurobiology
影响因子：
--
作者：
Yan,Q;Elliott,JL;Matheson,C;Sun,J;Zhang,L;Mu,X;Rex,KL;Snider,WD
通讯作者：
Snider,WD