Host-selected mutations converging on a global regulator drive an adaptive leap towards symbiosis in bacteria
Host-selected mutations converging on a global regulator drive an adaptive leap towards symbiosis in bacteria
复制标题
宿主选择的突变汇聚到全局调节器上，推动细菌向共生的适应性飞跃
作者：
M. S. Pankey;Randi L. Foxall;Ian M. Ster;L. Perry;B. M. Schuster;Rachel Donner;Matthew Coyle;V. Cooper;C. Whistler
宿主的免疫屏障和物理屏障可以抵御病原体，但也阻碍了基本共生伙伴关系的建立。为了揭示有益生物适应规避宿主防御的机制，我们通过实验进化出生态学上不同的生物发光弧菌fischeri的定殖和生长在鱿鱼Euprymna sputteropes的光器官内。细菌的连续鱿鱼传代在binK传感器激酶基因中产生了八个不同的突变，这赋予了一个特殊的选择性优势，可以通过经验和理论分析来证明。鱿鱼适应binK等位基因促进殖民化和免疫逃避介导的细胞相关基质，包括共生多糖（Syp）和纤维素。binK的变化也改变了群体感应，提高了发光诱导的阈值。BinK对共生性状的协调调节提供了一种有效的解决方案，其中改变BinK功能是解锁多种定殖障碍的关键。这些结果确定了微生物适应性的遗传基础，并强调了宿主作为塑造新兴共生体种群的选择剂的重要性。DOI：http://dx.doi.org/10.7554/eLife.24414.001网站
Host immune and physical barriers protect against pathogens but also impede the establishment of essential symbiotic partnerships. To reveal mechanisms by which beneficial organisms adapt to circumvent host defenses, we experimentally evolved ecologically distinct bioluminescent Vibrio fischeri by colonization and growth within the light organs of the squid Euprymna scolopes. Serial squid passaging of bacteria produced eight distinct mutations in the binK sensor kinase gene, which conferred an exceptional selective advantage that could be demonstrated through both empirical and theoretical analysis. Squid-adaptive binK alleles promoted colonization and immune evasion that were mediated by cell-associated matrices including symbiotic polysaccharide (Syp) and cellulose. binK variation also altered quorum sensing, raising the threshold for luminescence induction. Preexisting coordinated regulation of symbiosis traits by BinK presented an efficient solution where altered BinK function was the key to unlock multiple colonization barriers. These results identify a genetic basis for microbial adaptability and underscore the importance of hosts as selective agents that shape emergent symbiont populations. DOI: http://dx.doi.org/10.7554/eLife.24414.001
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影响因子：
5.1
作者：
Nyholm SV;Stewart JJ;Ruby EG;McFall-Ngai MJ
通讯作者：
McFall-Ngai MJ
影响因子：
15.9
作者：
Yildiz FH;Visick KL
通讯作者：
Visick KL
DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
Systems Biology
通讯作者：
Systems Biology
影响因子：
10.7
作者：
Jansen G;Crummenerl LL;Gilbert F;Mohr T;Pfefferkorn R;Thänert R;Rosenstiel P;Schulenburg H
通讯作者：
Schulenburg H
影响因子：
7
作者：
Bochner, BR;Gadzinski, P;Panomitros, E
通讯作者：
Panomitros, E