Efficient induction of transgene-free human pluripotent stem cells using a vector based on Sendai virus, an RNA virus that does not integrate into the host genome.
Efficient induction of transgene-free human pluripotent stem cells using a vector based on Sendai virus, an RNA virus that does not integrate into the host genome.
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DOI：
10.2183/pjab.85.348
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Hasegawa M
中科院分区：
文献类型：
--
作者：
Fusaki N;Ban H;Nishiyama A;Saeki K;Hasegawa M
诱导多能干细胞（iPSC）已经通过引入重编程因子从体细胞产生。将外源基因整合到宿主基因组中是临床应用的技术障碍。在这里，我们表明仙台病毒（SeV）是一种RNA病毒，没有改变宿主基因组的风险，是产生安全iPSC的有效解决方案。仙台病毒人类iPSC表达多能性基因，显示重编程细胞的去甲基化特征。SeV衍生的转基因在细胞分裂过程中减少。此外，病毒能够通过利用在SeV感染的细胞上表达的细胞表面标记HN的抗体介导的阴性选择容易地去除。无病毒iPSC在体内和体外分化为三个胚层的成熟细胞，包括搏动的心肌细胞、神经元、骨和胰腺细胞。我们的数据表明，高效，非整合SeV为基础的载体系统提供了一个关键的解决方案，重编程体细胞，并将加速临床应用。
Induced pluripotent stem cells (iPSC) have been generated from somatic cells by introducing reprogramming factors. Integration of foreign genes into the host genome is a technical hurdle for the clinical application. Here, we show that Sendai virus (SeV), an RNA virus and carries no risk of altering host genome, is an efficient solution for generating safe iPSC. Sendai-viral human iPSC expressed pluripotency genes, showed demethylation characteristic of reprogrammed cells. SeV-derived transgenes were decreased during cell division. Moreover, viruses were able to be easily removed by antibody-mediated negative selection utilizing cell surface marker HN that is expressed on SeV-infected cells. Viral-free iPSC differentiated to mature cells of the three embryonic germ layers in vivo and in vitro including beating cardiomyocytes, neurons, bone and pancreatic cells. Our data demonstrated that highly-efficient, non-integrating SeV-based vector system provides a critical solution for reprogramming somatic cells and will accelerate the clinical application.
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DOI：
10.1126/science.1162494
发表时间：
2008-11-07
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Stadtfeld M;Nagaya M;Utikal J;Weir G;Hochedlinger K
通讯作者：
Hochedlinger K
影响因子：
64.5
作者：
Soldner F;Hockemeyer D;Beard C;Gao Q;Bell GW;Cook EG;Hargus G;Blak A;Cooper O;Mitalipova M;Isacson O;Jaenisch R
通讯作者：
Jaenisch R
影响因子：
5.4
作者：
Harui, A;Suzuki, S;Mitani, K
通讯作者：
Mitani, K
影响因子：
64.8
作者：
Park, In-Hyun;Zhao, Rui;Daley, George Q.
通讯作者：
Daley, George Q.
影响因子：
23.9
作者：
Kim D;Kim CH;Moon JI;Chung YG;Chang MY;Han BS;Ko S;Yang E;Cha KY;Lanza R;Kim KS
通讯作者：
Kim KS