Colon-derived liver metastasis, colorectal carcinoma, and hepatocellular carcinoma can be discriminated by the Ca(2+)-binding proteins S100A6 and S100A11.
Colon-derived liver metastasis, colorectal carcinoma, and hepatocellular carcinoma can be discriminated by the Ca(2+)-binding proteins S100A6 and S100A11.
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结肠衍生的肝转移，结直肠癌和肝细胞癌可以通过Ca（2+） - 结合蛋白S100A6和S100A11区分。
DOI：
10.1371/journal.pone.0003767
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发表时间：
2008
期刊：
影响因子：
3.7
通讯作者：
von Eggeling, Ferdinand
中科院分区：
文献类型：
--
作者：
Melle, Christian;Ernst, Guenther;Schimmel, Bettina;Bleul, Annett;von Eggeling, Ferdinand
在蛋白质组学水平上，肿瘤的蛋白质模式是否在转移过程中发生变化，或者是否存在允许转移分配给特定肿瘤实体的标志物，目前尚不清楚。如果原发肿瘤未知，后者具有临床意义。在这项研究中，来自结肠源性肝转移的组织（n = 17）被分类，激光显微解剖，并通过蛋白质芯片阵列（SELDI）进行分析。结果光谱与我们以前研究的原发性结直肠癌（CRC）和肝细胞癌（HCC）的数据进行了比较。在原发性肝癌、原发性结直肠癌和肝转移中差异表达的49个信号中，有两个通过免疫清除被鉴定为S100A6和S100A11。这两种蛋白在细胞中通过免疫组织化学精确定位。S100A6和S100A11可以区分CRC和HCC两种原发肿瘤实体，而S100A6可以区分转移和HCC。这两种鉴定的蛋白都可以用来区分不同的肿瘤实体。不同原发癌症转移的特定标记物或蛋白质组学模式将使我们能够确定转移的生物学特征。目前尚不清楚肿瘤的蛋白质模式在转移过程中是如何变化的，也不清楚是否存在允许转移分配给特定肿瘤实体的标志物。如果原发肿瘤未知，后者具有临床意义。
It is unknown, on the proteomic level, whether the protein patterns of tumors change during metastasis or whether markers are present that allow metastases to be allocated to a specific tumor entity. The latter is of clinical interest if the primary tumor is not known. In this study, tissue from colon-derived liver metastases (n = 17) were classified, laser-microdissected, and analysed by ProteinChip arrays (SELDI). The resulting spectra were compared with data for primary colorectal (CRC) and hepatocellular carcinomas (HCC) from our former studies. Of 49 signals differentially expressed in primary HCC, primary CRC, and liver metastases, two were identified by immunodepletion as S100A6 and S100A11. Both proteins were precisely localized immunohistochemically in cells. S100A6 and S100A11 can discriminate significantly between the two primary tumor entities, CRC and HCC, whereas S100A6 allows the discrimination of metastases and HCC. Both identified proteins can be used to discriminate different tumor entities. Specific markers or proteomic patterns for the metastases of different primary cancers will allow us to determine the biological characteristics of metastasis in general. It is unknown how the protein patterns of tumors change during metastasis or whether markers are present that allow metastases to be allocated to a specific tumor entity. The latter is of clinical interest if the primary tumor is not known.
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影响因子：
7
作者：
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影响因子：
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