CTX-M-15-Producing E. coli Isolates from Food Products in Germany Are Mainly Associated with an IncF-Type Plasmid and Belong to Two Predominant Clonal E. coli Lineages.
CTX-M-15-Producing E. coli Isolates from Food Products in Germany Are Mainly Associated with an IncF-Type Plasmid and Belong to Two Predominant Clonal E. coli Lineages.
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DOI：
10.3389/fmicb.2017.02318
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发表时间：
2017
影响因子：
5.2
通讯作者：
Käsbohrer A
中科院分区：
文献类型：
--
作者：
Irrgang A;Falgenhauer L;Fischer J;Ghosh H;Guiral E;Guerra B;Schmoger S;Imirzalioglu C;Chakraborty T;Hammerl JA;Käsbohrer A
超广谱β-内酰胺酶（ESBL）介导的第三代头孢菌素耐药性是一个重大的公共卫生问题。由于食物可能是产ESLB细菌传播的媒介，因此展开了一项关于食物中出现头孢菌素耐药大肠杆菌的研究。从动物源性食品样品（例如，家禽产品、猪肉、牛肉和生奶）。CTX-M-15是产ESBL大肠杆菌中含量最高的酶。大肠杆菌引起人类感染，本研究的重点是E。从食物样本中分离出含有blaCTX-M-15基因的大肠杆菌。blaCTX-M-15基因的检出率为5.2%（n = 21）。分子分析揭示了一个系统发育组A ST 167克隆，该克隆在6个月的时间内从生牛奶和牛肉样品中反复分离。分析表明，产生CTX-M-15的大肠杆菌的传播。德国食品样本中的大肠杆菌与多复制子IncF（FIA FIB PHI）质粒和其他抗菌药物耐药性基因（例如aac（6）-Ib-cr、blaOXA-1、catB 3、不同的tet变体以及带有aadA的1类整合子）相关5/dfrA 17基因盒。此外，还检测到4株系统发育组A的ST 410分离株。其中3株携带blaCTX-M-15基因的染色体拷贝，1株在90 kb IncF质粒上携带该基因。blaCTX-M-15基因总是与ISEcp 1元件相关联。因此，产CTX-M-15的E.在德国的食物样本中发现大肠杆菌。在不同基质的分离物中，鉴定出两个突出的克隆谱系，即A-ST 167和A-ST 410。这些谱系可能是重要的食源性传播的CTX-M-15生产的E。德国的大肠杆菌。有趣的是，据报道这些克隆谱系广泛分布，特别是在人类和牲畜的分离株中。携带CTX-M-15的分离株从食用动物传播到食品似乎是可能的，因为在同一时间段内从牲畜和食品样本中获得的分离株与从人类中检测到的分离株表现出相当的特征。但传播途径和方向还需进一步调查。
Extended-spectrum beta-lactamases (ESBL) mediating resistance to 3rd generation cephalosporins are a major public health issue. As food may be a vehicle in the spread of ESLB-producing bacteria, a study on the occurrence of cephalosporin-resistantu Escherichia coli in food was initiated. A total of 404 ESBL-producing isolates were obtained from animal-derived food samples (e.g., poultry products, pork, beef and raw milk) between 2011 and 2013. As CTX-M-15 is the most abundant enzyme in ESBL-producing E. coli causing human infections, this study focusses on E. coli isolates from food samples harboring the blaCTX-M-15 gene. The blaCTX-M-15 gene was detected in 5.2% (n = 21) of all isolates. Molecular analyses revealed a phylogenetic group A ST167 clone that was repeatedly isolated from raw milk and beef samples over a period of 6 months. The analyses indicate that spread of CTX-M-15-producing E. coli in German food samples were associated with a multireplicon IncF (FIA FIB FII) plasmid and additional antimicrobial resistance genes such as aac(6)-Ib-cr, blaOXA−1, catB3, different tet-variants as well as a class 1 integron with an aadA5/dfrA17 gene cassette. In addition, four phylogenetic group A ST410 isolates were detected. Three of them carried a chromosomal copy of the blaCTX-M-15 gene and a single isolate with the gene on a 90 kb IncF plasmid. The blaCTX-M-15 gene was always associated with the ISEcp1 element. In conclusion, CTX-M-15-producing E. coli were detected in German food samples. Among isolates of different matrices, two prominent clonal lineages, namely A-ST167 and A-ST410, were identified. These lineages may be important for the foodborne dissemination of CTX-M-15-producing E. coli in Germany. Interestingly, these clonal lineages were reported to be widely distributed and especially prevalent in isolates from humans and livestock. Transmission of CTX-M-15-harboring isolates from food-producing animals to food appears probable, as isolates obtained from livestock and food samples within the same time period exhibit comparable characteristics as compared to isolates detected from human. However, the routes and direction of transmission need further investigation.
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影响因子：
3.1
作者：
NATARO, JP;SERIWATANA, J;MORRIS, JG
通讯作者：
MORRIS, JG
影响因子：
5.2
作者：
Fischer, Jennie;Rodriguez, Irene;Guerra, Beatriz
通讯作者：
Guerra, Beatriz
影响因子：
11.8
作者：
Liebana, Ernesto;Carattoli, Alessandra;Threlfall, John
通讯作者：
Threlfall, John
影响因子：
9.4
作者：
Joensen, Katrine Grimstrup;Scheutz, Flemming;Aarestrup, Frank M.
通讯作者：
Aarestrup, Frank M.
影响因子：
1.8
作者：
Ben Sallem, Rym;Ben Slama, Karim;Torres, Carmen
通讯作者：
Torres, Carmen