OMIP-070: NKp46-Based 27-Color Phenotyping to Define Natural Killer Cells Isolated From Human Tumor Tissues.
OMIP-070: NKp46-Based 27-Color Phenotyping to Define Natural Killer Cells Isolated From Human Tumor Tissues.
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DOI：
10.1002/cyto.a.24230
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发表时间：
2020-10
期刊：
影响因子：
--
通讯作者：
Prlic M
中科院分区：
文献类型：
--
作者：
Frutoso M;Mair F;Prlic M
该27色面板经过验证和优化，可使用基于II型胶原酶的消化方案全面分析从人类肿瘤中分离的自然杀伤（NK）细胞。我们证实，在组织消化过程中，通过我们的最终组中使用的抗体检测蛋白质表达不受影响。在该评价过程中，我们发现CD56（一种通常用于识别NK细胞的生物标志物）的检测受到基于胶原酶的消化的显著影响。因此，我们的组集中在NKp46的表达，其足以鉴定NK细胞并且不受组织胶原酶消化步骤的影响。我们的小组还包括用于外推NK细胞成熟，分化，迁移，归巢潜力和功能状态的生物标志物。我们的样本组旨在提供从组织中分离的人NK细胞的深入表征，我们专门使用口腔鳞状细胞癌组织进行了测试，但它与其他可以用基于II型胶原酶的方案解离的组织相容。版权所有2020作者。由Wiley Periodicals LLC代表International Society for Advancement of Cytometry出版的Cytometry Part A。 本OMIP描述了一种适用于表征从人体组织中分离的NK细胞的27色流式细胞术面板。针对与基于II型胶原酶消化的相容性，对该样本组进行了优化。
This 27‐color panel has been validated and optimized to comprehensively profile natural killer (NK) cells isolated from human tumors using a collagenase Type II‐based digestion protocol. We confirmed that detection of protein expression by antibodies used in our final panel was not affected during tissue digestion. During this evaluation process, we found that detection of CD56, a biomarker typically used to identify NK cells, was affected substantially by collagenase‐based digestion. Thus, our panel is centered around expression of NKp46, which is sufficient to identify NK cells and not affected by the tissue collagenase digestion step. Our panel further includes biomarkers used to extrapolate NK‐cell maturation, differentiation, migration, homing potential, and functional state. Our panel is intended to provide in‐depth characterization of human NK cells isolated from tissues, which we specifically tested using oral squamous cell carcinomas tissues, but it is compatible with other tissues that can be dissociated with a collagenase Type II‐based protocol. © 2020 The Authors. Cytometry Part A published by Wiley Periodicals LLC on behalf of International Society for Advancement of Cytometry. This OMIP describes a 27‐color flow cytometry panel suited to characterize NK cells isolated from human tissues. This panel was optimized for compatibility with collagenase type II‐based digestion.
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影响因子：
16.8
作者：
Bendall SC;Nolan GP;Roederer M;Chattopadhyay PK
通讯作者：
Chattopadhyay PK
影响因子：
15.9
作者：
Chockley, Peter J.;Chen, Jun;Keshamouni, Venkateshwar G.
通讯作者：
Keshamouni, Venkateshwar G.
影响因子：
4
作者：
Erokhina SA;Streltsova MA;Kanevskiy LM;Telford WG;Sapozhnikov AM;Kovalenko EI
通讯作者：
Kovalenko EI
影响因子：
5.4
作者：
KIESSLING, R;KLEIN, E;WIGZELL, H
通讯作者：
WIGZELL, H
DOI：
10.1084/jem.20052507
发表时间：
2006-04-17
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Freud AG;Yokohama A;Becknell B;Lee MT;Mao HC;Ferketich AK;Caligiuri MA
通讯作者：
Caligiuri MA