A deep profiler's guide to cytometry.
A deep profiler's guide to cytometry.
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DOI：
10.1016/j.it.2012.02.010
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发表时间：
2012-07
影响因子：
16.8
通讯作者：
Chattopadhyay PK
中科院分区：
文献类型：
--
作者：
Bendall SC;Nolan GP;Roederer M;Chattopadhyay PK
近年来，技术的进步为我们提供了量化单个细胞中多个基因表达的工具。它们在宿主中的功能。 。质谱的免疫表型可以以每秒1,000个细胞的速度测量超过三十个蛋白质。
In recent years, advances in technology have provided us with tools to quantify the expression of multiple genes in individual cells. The ability to simultaneously measure multiple genes on the same cell is necessary to resolve the incredible diversity of cell subsets, as well as to define their function in the host. Fluorescence-based flow cytometry is the benchmark for this; with it, we can quantify 18 proteins per cell, at >10,000 cells per second. “Mass cytometry” is a new technology that promises to significantly extend these capabilities. Immunophenotyping by mass spectrometry provides the ability to measure more than three dozen proteins at a rate of 1,000 cells per second. We review these cytometric technologies, capable of high-content, high-throughput single-cell assays.
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DOI：
10.1126/science.1198704
发表时间：
2011-05-06
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Bendall SC;Simonds EF;Qiu P;Amir el-AD;Krutzik PO;Finck R;Bruggner RV;Melamed R;Trejo A;Ornatsky OI;Balderas RS;Plevritis SK;Sachs K;Pe'er D;Tanner SD;Nolan GP
通讯作者：
Nolan GP
影响因子：
82.9
作者：
Gattinoni L;Lugli E;Ji Y;Pos Z;Paulos CM;Quigley MF;Almeida JR;Gostick E;Yu Z;Carpenito C;Wang E;Douek DC;Price DA;June CH;Marincola FM;Roederer M;Restifo NP
通讯作者：
Restifo NP
影响因子：
20.3
作者：
Borsellino, Giovanna;Kleinewietfeld, Markus;Falk, Kirsten
通讯作者：
Falk, Kirsten
DOI：
10.1007/978-1-61737-950-5_2
发表时间：
2011-01-01
期刊：
FLOW CYTOMETRY PROTOCOLS, THIRD EDITION
影响因子：
--
作者：
Bagwell, C. Bruce
通讯作者：
Bagwell, C. Bruce
影响因子：
7.4
作者：
Baranov, VI;Quinn, Z;Tanner, SD
通讯作者：
Tanner, SD