Molecular characterization of a novel Staphylococcus aureus surface protein (SasC) involved in cell aggregation and biofilm accumulation.
Molecular characterization of a novel Staphylococcus aureus surface protein (SasC) involved in cell aggregation and biofilm accumulation.
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DOI：
10.1371/journal.pone.0007567
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发表时间：
2009-10-23
期刊：
影响因子：
3.7
通讯作者：
Heilmann C
中科院分区：
文献类型：
--
作者：
Schroeder K;Jularic M;Horsburgh SM;Hirschhausen N;Neumann C;Bertling A;Schulte A;Foster S;Kehrel BE;Peters G;Heilmann C
葡萄球菌是引起植入物相关感染的最重要的病原体。通过形成由细菌和宿主材料（称为生物膜）组成的三维结构，植入医疗器械的定植被认为是这些感染中最关键的因素。为了形成生物膜，细菌首先附着在医疗器械的表面，然后增殖并积累成多层细胞簇。生物膜的积累可能是由多糖和蛋白质因子介导的。关于金黄色葡萄球菌蛋白因子参与生物膜积累的信息有限，因此，我们搜索了金黄色葡萄球菌。aureus Col基因组中含有LPXTG基序的潜在表面蛋白，并选择了迄今为止尚未表征的S.金黄色葡萄球菌表面蛋白C（SasC）用于进一步研究。SasC序列由2186个氨基酸组成，分子量为238 kDa，具有革兰氏阳性表面蛋白的典型特征，如N端信号肽、C端LPXTG细胞壁锚定基序和一个由17个重复序列组成的重复区，与功能未知结构域1542（DUF 1542）相似。我们在肉葡萄球菌中异源表达sasC，这导致形成巨大的细胞聚集体，表明细胞间粘附和生物膜积累。为了定位赋予细胞聚集的结构域，我们表达了两个sasC亚克隆，其编码包括在各种结构（FIVAR）中发现的基序的N-末端结构域或8个DUF 1542重复。SasC或其N-末端结构域，而不是DUF 1542重复区赋予巨大的细胞聚集体的产生，更高的附着到聚苯乙烯，并增强生物膜的形成S。carnosus和S.金黄色。SasC不介导与纤维蛋白原、血小板反应蛋白-1、血管性血友病因子或血小板的结合，如通过流式细胞术测定的。因此，SasC代表了一个新的S.金黄色葡萄球菌蛋白因子参与细胞聚集和生物膜形成，可能在该重要病原体感染过程中的定殖中起重要作用。
Staphylococci belong to the most important pathogens causing implant-associated infections. Colonization of the implanted medical devices by the formation of a three-dimensional structure made of bacteria and host material called biofilm is considered the most critical factor in these infections. To form a biofilm, bacteria first attach to the surface of the medical device, and then proliferate and accumulate into multilayered cell clusters. Biofilm accumulation may be mediated by polysaccharide and protein factors. The information on Staphylococcus aureus protein factors involved in biofilm accumulation is limited, therefore, we searched the S. aureus Col genome for LPXTG-motif containing potential surface proteins and chose the so far uncharacterized S. aureus surface protein C (SasC) for further investigation. The deduced SasC sequence consists of 2186 amino acids with a molecular mass of 238 kDa and has features typical of Gram-positive surface proteins, such as an N-terminal signal peptide, a C-terminal LPXTG cell wall anchorage motif, and a repeat region consisting of 17 repeats similar to the domain of unknown function 1542 (DUF1542). We heterologously expressed sasC in Staphylococcus carnosus, which led to the formation of huge cell aggregates indicative of intercellular adhesion and biofilm accumulation. To localize the domain conferring cell aggregation, we expressed two subclones of sasC encoding either the N-terminal domain including a motif that is found in various architectures (FIVAR) or 8 of the DUF1542 repeats. SasC or its N-terminal domain, but not the DUF1542 repeat region conferred production of huge cell aggregates, higher attachment to polystyrene, and enhanced biofilm formation to S. carnosus and S. aureus. SasC does not mediate binding to fibrinogen, thrombospondin-1, von Willebrand factor, or platelets as determined by flow cytometry. Thus, SasC represents a novel S. aureus protein factor involved in cell aggregation and biofilm formation, which may play an important role in colonization during infection with this important pathogen.
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Pfam：氏族、网络工具和服务。
影响因子：
14.9
作者：
Finn, Robert D.;Mistry, Jaina;Schuster-Bockler, Benjamin;Griffiths-Jones, Sam;Hollich, Volker;Lassmann, Timo;Moxon, Simon;Marshall, Mhairi;Khanna, Ajay;Durbin, Richard;Eddy, Sean R.;Sonnhammer, Erik L. L.;Bateman, Alex
通讯作者：
Bateman, Alex
影响因子：
3.1
作者：
Beenken, KE;Blevins, JS;Smeltzer, MS
通讯作者：
Smeltzer, MS
影响因子：
3.1
作者：
Cramton, SE;Gerke, C;Götz, F
通讯作者：
Götz, F
影响因子：
20.3
作者：
Dörmann, D;Clemetson, KJ;Kehrel, BE
通讯作者：
Kehrel, BE
影响因子：
3.6
作者：
Heilmann, C;Schweitzer, O;Gotz, F
通讯作者：
Gotz, F