Year-round reproduction and induced spawning of Chinese amphioxus, Branchiostoma belcheri, in laboratory.
Year-round reproduction and induced spawning of Chinese amphioxus, Branchiostoma belcheri, in laboratory.
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中国文昌鱼（Branchiostoma belcheri）的实验室全年繁殖和诱导产卵。
DOI：
10.1371/journal.pone.0075461
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Li G;Shu Z;Wang Y
文昌鱼是研究脊椎动物进化和发育机制的最佳候选者，因为它具有类似于脊椎动物但更简单的形态、胚胎发育和基因组结构。全年生产活文昌鱼胚胎是比较进化和发育研究的理想选择。然而，所有文昌鱼物种在野外和实验室都有不同的繁殖季节。我们最近发现中国文昌鱼 B。在适当的条件下饲养，贝氏花金龟在自然繁殖期过后仍能重复繁殖。在这项研究中，我们能够进一步扩展并在2011年10月至2012年10月期间全年生产胚胎。结果发现，所有被检动物在被检期间均重复产卵。此外，两种文昌鱼 B。贝尔切里和 B。日本文昌鱼可以用热休克法诱导产卵，但诱导产卵的效率不如欧洲文昌鱼。总的来说，我们成功地生产了 B。贝氏胚胎几乎每天全年。这一进展将为文昌鱼的进化和发育研究提供必要的胚胎材料，并对其他文昌鱼的养殖和产卵诱导具有重要意义。
Amphioxus is a best candidate for studying the evolutionary and developmental mechanisms of vertebrates, because of its vertebrate-like but much simpler morphology, embryonic development and genome structure. Producing live amphioxus embryos throughout the year is an ideal for comparative evolution and developmental studies. However, all amphioxus species have distinct breeding seasons in the wild and laboratory. We recently found that Chinese amphioxus B . belcheri could reproduce repeatedly beyond its natural breeding season when reared under proper conditions. In this study, we were able to extend further and produce embryos throughout the year from October 2011 to October 2012. We found all examined animals had spawned repeatedly during the examined period. In addition, both lancelets B . belcheri and B . japonicum could be induced to spawn by heat-shock method, although the induced spawning efficiency was not as high as that observed in the European lancelet. In general, we have succeeded in producing B . belcheri embryos almost daily throughout the year. This advancement will provide essential embryonic material for evolutionary and developmental studies, and have great implications for the cultivation and spawning induction of other amphioxus species.
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影响因子：
64.8
作者：
GARCIAFERNANDEZ, J;HOLLAND, PWH
通讯作者：
HOLLAND, PWH
影响因子：
3.7
作者：
Li G;Yang X;Shu Z;Chen X;Wang Y
通讯作者：
Wang Y
影响因子：
64.8
作者：
Delsuc, F;Brinkmann, H;Philippe, H
通讯作者：
Philippe, H
DOI：
10.1002/jez.b.21179
发表时间：
2007-07-15
影响因子：
2.2
作者：
Fuentes, Michael;Benito, Elia;Escriva, Hector
通讯作者：
Escriva, Hector
影响因子：
1.6
作者：
HOLLAND, ND;HOLLAND, LZ
通讯作者：
HOLLAND, LZ