Consecutive spawnings of Chinese amphioxus, Branchiostoma belcheri, in captivity.
Consecutive spawnings of Chinese amphioxus, Branchiostoma belcheri, in captivity.
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DOI：
10.1371/journal.pone.0050838
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Li G;Yang X;Shu Z;Chen X;Wang Y
文昌鱼因其独特的系统发育位置、简单的体型和基因组测序而成为研究脊椎动物进化和发育机制的有前景的模型动物。然而，使用文昌鱼作为模式生物的一个主要缺点是活胚胎的供应有限，因为它们只能在产卵季节获得，根据物种的不同，产卵季节从几天到几个月不等。因此，我们的目标是开发在非产卵季节获得有活力的文昌鱼胚胎的方法。在目前的研究中，我们发现，在实验室中，在高温（25-28°C）低密度培养、提供充足的食物和适当的清洁度的情况下，Branchiostoma belceri可以发育其性腺并连续产卵。在2011年11月至12月观察到的约150只自然产卵动物中，截至2012年4月，10%的动物产卵过两次，10%的动物产卵过三次，80%的动物产过四次。连续产卵的配子质量和数量与自然产卵的配子质量和数量没有明显差异。不同动物之间的产卵间隔（1 至 5 个月）和单个个体之间的产卵间隔（一只动物为 27 至 74 天）之间差异很大。总之，我们开发了一种方法，首次可以实现圈养文昌鱼的连续产卵。这对其他文昌鱼物种的培育具有实际意义，最终将极大地促进文昌鱼作为模式系统的利用。
Cephalochordate amphioxus is a promising model animal for studying the evolutionary and developmental mechanisms of vertebrates because its unique phylogenetic position, simple body plan and sequenced genome. However, one major drawback for using amphioxus as a model organism is the restricted supply of living embryos since they are available only during spawning season that varies from a couple of days to several months according to species. Therefore we are aiming to develop methods for obtaining viable amphioxus embryos in non-spawning season. In the current study, we found that Branchiostoma belcheri could develop their gonads and spawn consecutively in the laboratory when cultured in a low density at a high temperature (25–28°C) supplied with sufficient food and proper cleanness. Among the approximate 150 observed animals, which spawned spontaneously between November and December 2011, 10% have spawned twice, 10% three times, and 80% four times, through April 2012. The quality and quantity of the gametes reproduced in the consecutive spawning have no obvious difference with those spawned once naturally. Spawning intervals varied dramatically both among different animals (from 1 to 5 months) and between intervals of a single individual (from 27 to 74 days for one animal). In summary, we developed a method with which, for the first time, consecutive spawnings of amphioxus in captivity can be achieved. This has practical implications for the cultivation of other amphioxus species, and eventually will greatly promote the utilization of amphioxus as a model system.
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影响因子：
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作者：
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通讯作者：
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DOI：
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发表时间：
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影响因子：
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作者：
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