Measurement of cytokine biomarkers using an aptamer-based affinity graphene nanosensor on a flexible substrate toward wearable applications.
Measurement of cytokine biomarkers using an aptamer-based affinity graphene nanosensor on a flexible substrate toward wearable applications.
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DOI：
10.1039/c8nr04315a
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发表时间：
2018-11-29
期刊：
影响因子：
6.7
通讯作者：
Lin Q
中科院分区：
文献类型：
--
作者：
Hao Z ;Wang Z ;Li Y ;Zhu Y ;Wang X ;De Moraes CG ;Pan Y ;Zhao X ;Lin Q
我们提出了一种无标记检测细胞因子生物标志物的方法，该方法使用适体功能化的石墨烯基场效应晶体管（GFET）纳米传感器，该传感器位于聚合物聚萘二甲酸乙二醇酯（PEN）的柔性SiO2涂层基底上。纳米传感器符合下面的非平面表面，并进行适体-生物标志物结合的基于GFET的快速转导，从而潜在地允许检测从人类体液可靠地采样的细胞因子生物标志物（例如，汗）在可穿戴传感应用中。在表征可穿戴应用的纳米传感器的适用性，我们调查的适体和生物标志物以及石墨烯纳米粒子之间的平衡解离常数的基板弯曲的影响。通过检测肿瘤坏死因子-α（TNF-α），一种炎症细胞因子生物标志物，证明了纳米传感器的实用性。实验结果表明，柔性纳米传感器可以在5分钟内特异性地响应TNF-α浓度的变化，检测限低至26 pM，具有可重复性。
We present an approach to label-free detection of cytokine biomarkers using an aptamer-functionalized, graphene-based field effect transistor (GFET) nanosensor on a flexible, SiO2-coated substrate of the polymer polyethylene naphthalate (PEN). The nanosensor conforms to the underlying nonplanar surface and performs GFET-based rapid transduction of the aptamer-biomarker binding, thereby potentially allowing detection of cytokine biomarkers that are sampled reliably from human bodily fluids (e.g., sweat) in wearable sensing applications. In characterizing the suitability of the nanosensor for wearable applications, we investigate the effects of the substrate bending on the equilibrium dissociation constant between the aptamer and the biomarker as well as the graphene transconductance. The utility of the nanosensor is demonstrated by the detection of tumor necrosis factor-α (TNF-α), an inflammatory cytokine biomarker. Experimental results show that the flexible nanosensor can specifically respond to changes in the TNF-α concentration within 5 minutes with a limit of detection as low as 26 pM in a repeatable manner.
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