Characterization and developmental expression of AmphiMef2 gene in amphioxus
Characterization and developmental expression of AmphiMef2 gene in amphioxus
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文昌鱼 AmphiMef2 基因的特征和发育表达
DOI：
10.1007/s11427-007-0082-5
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发表时间：
2007-10
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影响因子：
--
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中科院分区：
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--
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肌细胞增强因子2蛋白是MADS转录因子家族的成员，可以控制脊椎动物肌肉特异性基因的表达。然而，并不是所有的Mef2基因都对无脊椎动物的肌肉发育至关重要。在这里，我们从文昌鱼中分离出一个全长cDNA，命名为AmphiMef2。预测的氨基酸序列具有高度保守的MADS和MEF2结构域，在脊椎动物中比在无脊椎动物中与其同源物的对应区域具有更高的一致性。全载原位杂交结果表明，在神经发育早期，AmphiMef2首先在体前中胚层中表达，然后在体前和未分节的体前中胚层中都能检测到转录本。在幼虫期36 h，仅在后体中检测到该表达。在幼虫期48 h，表达转移到口前窝（脊椎动物腺垂体的同源器官），并持续到至少72 h幼虫期。结果提示，amphime2可能不仅参与文昌鱼的肌生成，还参与了口前窝的发育或功能。
Myocyte enhancer factor 2 proteins are members of MADS family of transcription factors, which can control the expression of muscle-specific genes in vertebrates. However, not all Mef2 genes are essential for muscle development in invertebrates. Here we have isolated a full-length cDNA from amphioxus, designated AmphiMef2. The predicted amino acid sequence has highly conserved MADS and MEF2 domains, showing higher identity with the corresponding regions of its homologues in vertebrates than those in invertebrates. Results from whole-mount in situ hybridization show that the expression of AmphiMef2 initially appears in the presomitic mesoderm at early neurula stage, then the transcripts are detected in both the somites and the unsegmented presomitic mesoderm. At 36 h larval stage, the expression is only detected in the posterior somites. By 48 h larval stage, the expression is shifted to the preoral pit (a homologous organ to the vertebrate adenohypophysis) and persists until at least 72 h larval stage. The results suggest that AmphiMef2 may be not only involved in the myogenesis but also the development or function of the preoral pit in amphioxus.
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影响因子：
2.7
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