PiRamid: A compact Raspberry Pi imaging box to automate small-scale time-lapse digital analysis, suitable for laboratory and field use.
PiRamid: A compact Raspberry Pi imaging box to automate small-scale time-lapse digital analysis, suitable for laboratory and field use.
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DOI：
10.1016/j.ohx.2022.e00377
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发表时间：
2022-10
期刊：
影响因子：
2.2
通讯作者：
Edwards, Alexander Daniel
中科院分区：
文献类型：
--
作者：
Long, Matthew Michael;Diep, Tai The;Needs, Sarah Helen;Ross, Marta Joan;Edwards, Alexander Daniel
数字成像允许许多实验的定量，例如微生物生长分析，但是实验室数字成像系统可能昂贵且过于专业化。树莓派相机平台使自动化，控制成像负担得起，可访问的定制。当与开源软件和开源3D打印硬件相结合时，该平台对图像质量和捕获的控制允许新型仪器的快速发展。在这里，我们提出了“PiRamid”，一种紧凑，便携，廉价的外壳，用于实验室和现场的自主成像。模块化的三件式3D打印设计使其易于合并不同的相机系统或照明配置（例如，单波长的荧光LED）。与传统的数码相机或智能手机不同，这种封闭的设计允许在环境照明下完全控制照明，而不是在三脚架或手持设备上。可堆叠设计允许快速样品添加或相机焦点调整，在放大倍率和分辨率相应的变化。整个装置足够小，可以放入微生物培养箱中，并且足够便宜（约100英镑），可以扩展到更大的并行实验。简单地说，Python脚本完全自动化照明和图像捕获的小规模实验，面积为~ 110×85 mm，分辨率为70-90 μ m。我们展示了PiRamid的多功能性通过捕获时间分辨，定量的图像数据，广泛的分析。捕获常规微生物学（琼脂培养皿）、3D打印定制微生物实验室和微流体微生物学的细菌生长动力学。为了说明微生物学以外的应用，我们展示了沙拉叶晶体生长和降解的延时成像。微小的修改允许外延照明，增加一个LED环的相机模块。我们的结论是，PiRamid允许廉价的数字捕获和定量的大范围的实验通过延时成像，以简化实验室和现场成像。
Digital imaging permits the quantitation of many experiments, such as microbiological growth assays, but laboratory digital imaging systems can be expensive and too specialised. The Raspberry Pi camera platform makes automated, controlled imaging affordable with accessible customisation. When combined with open source software and open-source 3D printed hardware, the control over image quality and capture of this platform permits the rapid development of novel instrumentation. Here we present “PiRamid”, a compact, portable, and inexpensive enclosure for autonomous imaging both in the laboratory and in the field. The modular three-piece 3D printed design makes it easy to incorporate different camera systems or lighting configurations (e.g., single wavelength LED for fluorescence). The enclosed design allows complete control of illumination unlike a conventional digital camera or smartphone, on a tripod or handheld, under ambient lighting. The stackable design permits rapid sample addition or camera focus adjustment, with a corresponding change in magnification and resolution. The entire unit is small enough to fit within a microbiological incubator, and cheap enough (∼£100) to scale out for larger parallel experiments. Simply, Python scripts fully automate illumination and image capture for small-scale experiments with an ∼110×85 mm area at 70–90 µm resolution. We demonstrate the versatility of PiRamid by capturing time-resolved, quantitative image data for a wide range of assays. Bacterial growth kinetics was captured for conventional microbiology (agar Petri dishes), 3D printed custom microbiology labware and microfluidic microbiology. To illustrate application beyond microbiology, we demonstrate time-lapse imaging of crystal growth and degradation of salad leaves. Minor modifications permit epi-illumination by addition of a LED ring to the camera module. We conclude that PiRamid permits inexpensive digital capture and quantitation of a wide range of experiments by time-lapse imaging to simplify both laboratory and field imaging.
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影响因子：
3.7
作者：
Lu Q;Liu G;Xiao C;Hu C;Zhang S;Xu RX;Chu K;Xu Q;Smith ZJ
通讯作者：
Smith ZJ
DOI：
10.3390/medsci9010014
发表时间：
2021-02-21
期刊：
Medical sciences (Basel, Switzerland)
影响因子：
--
作者：
Kasimanickam V;Kasimanickam M;Kasimanickam R
通讯作者：
Kasimanickam R
影响因子：
3.9
作者：
Mohamed, Ashraf A.;Shalaby, Ahmed A.;Salem, Abdelnaby M.
通讯作者：
Salem, Abdelnaby M.
影响因子：
2.7
作者：
Neches, Russell Y.;Flynn, Kaitlin J.;Pudlo, Nicholas
通讯作者：
Pudlo, Nicholas
影响因子：
4.4
作者：
Deb, D.;Hariharan, S.;Ryu, Chang-Ha
通讯作者：
Ryu, Chang-Ha