A modular, open-source, slide-scanning microscope for diagnostic applications in resource-constrained settings.
A modular, open-source, slide-scanning microscope for diagnostic applications in resource-constrained settings.
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DOI：
10.1371/journal.pone.0194063
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Smith ZJ
中科院分区：
文献类型：
--
作者：
Lu Q;Liu G;Xiao C;Hu C;Zhang S;Xu RX;Chu K;Xu Q;Smith ZJ
在本文中，我们报告了一个具有成本效益的，模块化的，开源的，全自动化的幻灯片扫描显微镜，完全由容易获得的现成的部分，并能够明场和荧光模式的发展。自动化X-Y载物台由两个低成本的千分尺载物台组成，该载物台连接到以开环模式运行的步进电机。该显微镜由低成本CMOS传感器和低成本板透镜组成，放置在4f配置中。该系统具有约1微米的分辨率，受到可用板透镜的f/#的限制。该显微镜结构紧凑，尺寸仅为25×25×30 cm，X和Y方向的绝对定位精度为±1 μm。Z工作台可在大视场范围内自动对焦和成像，即使是在非平面样品上，定制软件可自动确定样品边界和图像拼接。我们通过对含有人类和动物寄生虫的荧光和透射染料染色的血液和粪便涂片以及几种制备的组织样本进行成像来证明我们的设备的实用性。这些结果表明，图像质量与高端商业显微镜相当，明场系统的成本不到400美元，荧光模块需要额外的100美元。
In this paper we report the development of a cost-effective, modular, open source, and fully automated slide-scanning microscope, composed entirely of easily available off-the-shelf parts, and capable of bright field and fluorescence modes. The automated X-Y stage is composed of two low-cost micrometer stages coupled to stepper motors operated in open-loop mode. The microscope is composed of a low-cost CMOS sensor and low-cost board lenses placed in a 4f configuration. The system has approximately 1 micron resolution, limited by the f/# of available board lenses. The microscope is compact, measuring just 25×25×30 cm, and has an absolute positioning accuracy of ±1 μm in the X and Y directions. A Z-stage enables autofocusing and imaging over large fields of view even on non-planar samples, and custom software enables automatic determination of sample boundaries and image mosaicking. We demonstrate the utility of our device through imaging of fluorescent- and transmission-dye stained blood and fecal smears containing human and animal parasites, as well as several prepared tissue samples. These results demonstrate image quality comparable to high-end commercial microscopes at a cost of less than US$400 for a bright-field system, with an extra US$100 needed for the fluorescence module.
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平铺 3D 显微图像采集的全局最佳拼接。
DOI：
10.1093/bioinformatics/btp184
发表时间：
2009-06-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Preibisch S;Saalfeld S;Tomancak P
通讯作者：
Tomancak P
影响因子：
6.1
作者：
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通讯作者：
Ozcan, Aydogan
影响因子：
3.2
作者：
Absar, Syeda Fatima;Tahir, Mohammad;Wilbur, David
通讯作者：
Wilbur, David
影响因子：
6.2
作者：
Lau, KT;Baldwin, S;Diamond, D
通讯作者：
Diamond, D
影响因子：
2.7
作者：
Kosinski, Karen C.;Bosompem, Kwabena M.;Gute, David M.
通讯作者：
Gute, David M.