Photochemistry and Cytotoxicity Evaluation of Heptamethinecyanine Near Infrared (NIR) Dyes.
Photochemistry and Cytotoxicity Evaluation of Heptamethinecyanine Near Infrared (NIR) Dyes.
复制标题
红外红外（NIR）染料的光化学和细胞毒性评估。
DOI：
10.3390/ijms140918557
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发表时间：
2013-09-09
影响因子：
5.6
通讯作者：
Ferreira LF
中科院分区：
文献类型：
--
作者：
Conceição DS;Ferreira DP;Ferreira LF
本研究考察了潜在的光动力治疗光敏剂，即四种商品化的七氢呋喃染料(IR125、IR780、IR813、IR820)的光化学性质。用激光诱导荧光和激光闪光光解的方法进行了光谱研究，获得了荧光量子产率和瞬时吸收光谱。测定了它们的荧光寿命。所遇到的差异基本上与磺酸基与溶剂的相互作用有关，也与连接两个含氮杂环基团的中心桥的刚性有关。在充气和无氧的样品中进行了瞬时吸收研究，得出了光异构体和三线态的形成结论。以HeLa细胞为模型细胞，对所研究的四种染料在黑暗中和照射后的细胞毒性进行了评价，发现照射后细胞毒性发生了显著变化，主要是IR125和IR813染料。共聚焦显微镜分析显示，这些染料倾向于进入细胞内空间。
The present study investigates the photochemical properties of potential photosensitizers for photodynamic therapy, namely four commercial heptamethinecyanine dyes (IR125, IR780, IR813, IR820). Spectroscopic studies were made by means of laser induced fluorescence and laser flash photolysis in order to obtain fluorescence quantum yields and transient absorption spectra. Fluorescence lifetimes were also determined. The differences encountered were essentially related with the interaction of the sulfonate groups with the solvent, and also regarding the rigidification of the central bridge connecting the two nitrogen-containing heterocyclic groups. Transient absorption studies were performed both in aerated and oxygen free samples, to conclude about the formation of photoisomers and triplet state. For the four dyes under study, a cytotoxic evaluation in the dark and after irradiation was performed using HeLa cells as the model cell line, which revealed significant changes after irradiation mainly in IR125 and IR813 dyes. Confocal microscopy analysis showed that these dyes tend to enter to the intracellular space.
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影响因子：
3.3
作者：
Khairutdinov, RF;Serpone, N
通讯作者：
Serpone, N
DOI：
10.1016/s1011-1344(02)00325-1
发表时间：
2002-08-01
影响因子：
5.4
作者：
Kassab, K
通讯作者：
Kassab, K
DOI：
10.1016/1011-1344(88)85004-8
发表时间：
1988-05-01
影响因子：
5.4
作者：
HOEBEKE, M;SERET, A;VANDEVORST, A
通讯作者：
VANDEVORST, A
一种近红外七次甲基染料，具有内在的癌症靶向、成像和光敏特性
影响因子：
14
作者：
Tan, Xu;Luo, Shenglin;Shi, Chunmeng
通讯作者：
Shi, Chunmeng
影响因子：
3.4
作者：
Lo, Pui-Chi;Fong, Wing-Ping;Ng, Dennis K. P.
通讯作者：
Ng, Dennis K. P.