Cohesin is dispensable for centromere cohesion in human cells.
Cohesin is dispensable for centromere cohesion in human cells.
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DOI：
10.1371/journal.pone.0000318
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发表时间：
2007-03-28
期刊：
影响因子：
3.7
通讯作者：
Clarke, Duncan J.
中科院分区：
文献类型：
--
作者：
Diaz-Martinez, Laura A.;Gimenez-Abian, Juan F.;Clarke, Duncan J.
适当地调节人类染色体着丝粒的凝聚力对于准确的基因组传递是至关重要的。究竟凝聚力是如何维持的，然后在后期如何分解，目前还不清楚。我们研究了着丝粒粘附素复合体在人类细胞中的作用，通过使用RNA干扰来耗尽粘附素亚基，也通过表达不可切割的Rad21粘附素蛋白。Rad21缺失导致后期异常，在此期间姐妹染色单体以不同步的方式分离和分离。然而，在Rad21缺失的细胞中，着丝粒凝聚力在后期之前保持不变，着丝粒的初级收缩与对照细胞没有区别。不可切割的Rad21(NC-Rad21)的表达使正常被分离酶切割的位点发生突变，导致姐妹染色单体在前期和中期的延迟分辨，并在后期阻碍了染色体臂的分离，但不妨碍着丝粒分离。这些数据表明，在有丝分裂早期，粘附素复合体对于姐妹凝聚是必不可少的，但在姐妹分离和后期分离的保真度方面起着重要作用。RAD21分离酶敏感部位的裂解对臂分离很重要，但对着丝粒分离不重要。
Proper regulation of the cohesion at the centromeres of human chromosomes is essential for accurate genome transmission. Exactly how cohesion is maintained and is then dissolved in anaphase is not understood. We have investigated the role of the cohesin complex at centromeres in human cells both by depleting cohesin subunits using RNA interference and also by expressing a non-cleavable version of the Rad21 cohesin protein. Rad21 depletion results in aberrant anaphase, during which the sister chromatids separate and segregate in an asynchronous fashion. However, centromere cohesion was maintained before anaphase in Rad21-depleted cells, and the primary constrictions at centromeres were indistinguishable from those in control cells. Expression of non-cleavable Rad21 (NC-Rad21), in which the sites normally cleaved by separase are mutated, resulted in delayed sister chromatid resolution in prophase and prometaphase, and a blockage of chromosome arm separation in anaphase, but did not impede centromere separation. These data indicate that cohesin complexes are dispensable for sister cohesion in early mitosis, yet play an important part in the fidelity of sister separation and segregation during anaphase. Cleavage at the separase-sensitive sites of Rad21 is important for arm separation, but not for centromere separation.
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影响因子：
3.7
作者：
Díaz-Martínez LA;Giménez-Abián JF;Azuma Y;Guacci V;Giménez-Martín G;Lanier LM;Clarke DJ
通讯作者：
Clarke DJ
影响因子：
64.8
作者：
DOWNES, CS;CLARKE, DJ;JOHNSON, RT
通讯作者：
JOHNSON, RT
影响因子：
16
作者：
Huang, J;Hsu, JM;Laurent, BC
通讯作者：
Laurent, BC
影响因子：
4
作者：
Kenney, Renee Deehan;Heald, Rebecca
通讯作者：
Heald, Rebecca
影响因子：
56.9
作者：
Hauf, S;Waizenegger, IC;Peters, JM
通讯作者：
Peters, JM