Campylobacter jejuni ferric-enterobactin receptor CfrA is TonB3 dependent and mediates iron acquisition from structurally different catechol siderophores.
Campylobacter jejuni ferric-enterobactin receptor CfrA is TonB3 dependent and mediates iron acquisition from structurally different catechol siderophores.
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DOI：
10.1039/c3mt20254b
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发表时间：
2013-08
期刊：
影响因子：
--
通讯作者：
Stintzi A
中科院分区：
文献类型：
--
作者：
Naikare H;Butcher J;Flint A;Xu J;Raymond KN;Stintzi A
空肠弯曲杆菌NCTC 11168本身不产生任何内源性铁载体，但需要CfrA肠杆菌素转运蛋白用于体内定殖。此外，还对C. jejuni NCTC 11168含有三种不同的TonB能量转导系统，命名为TonB 1、TonB 2和TonB 3，尚未测试它们在铁载体摄取中的作用或它们的功能冗余。我们证明了C. jejuni NCTC 11168以能量依赖性方式转运铁肠杆菌素，需要TonB 3才能发挥完全活性，而TonB 1则表现出部分功能冗余。此外，C.空肠NCTC 11168在生长过程中可以利用多种结构不同的儿茶酚铁载体作为唯一的铁源。这种生长仅依赖于CfrA肠杆菌素转运蛋白，并突出了这种转运蛋白可以识别的广泛底物。TonB3也是大多数儿茶酚铁载体生长所必需的。此外，TonB1或TonB3足以在氯化血红素或血红蛋白上生长作为唯一的铁源，证明TonB1和TonB3之间的功能冗余。同基因缺失突变体的体内定殖试验表明，在该模型中，TonB2突变体的鸡定殖需要TonB1和TonB3。这些结果进一步强调了铁转运对高效C.空肠定植。
Campylobacter jejuni NCTC11168 does not produce any endogenous siderophores of its own yet requires the CfrA enterobactin transporter for in vivo colonization. In addition, the genome of C. jejuni NCTC11168 contains three distinct TonB energy transduction systems, named TonB1, TonB2, and TonB3, that have not been tested for their role in siderophore uptake or their functional redundancy. We demonstrate that C. jejuni NCTC11168 transports ferric-enterobactin in an energy dependant manner that requires TonB3 for full activity with TonB1 showing partial functional redundancy. Moreover C. jejuni NCTC11168 can utilize a wide variety of structurally different catechol siderophores as sole iron sources during growth. This growth is solely dependent on the CfrA enterobactin transporter and highlights the wide range of substrates that this transporter can recognize. TonB3 is also required for growth on most catechol siderophores. Furthermore, either TonB1 or TonB3 is sufficient for growth on hemin or hemoglobin as a sole iron source demonstrating functional redundancy between TonB1 and TonB3. In vivo colonization assays with isogenic deletion mutants revealed that both TonB1 and TonB3 are required for chick colonization with TonB2 dispensable in this model. These results further highlight the importance of iron transport for efficient C. jejuni colonization.
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影响因子：
3.7
作者：
Blanvillain S;Meyer D;Boulanger A;Lautier M;Guynet C;Denancé N;Vasse J;Lauber E;Arlat M
通讯作者：
Arlat M
影响因子：
3.1
作者：
PICKETT, CL;AUFFENBERG, T;JUSUF, SSD
通讯作者：
JUSUF, SSD
影响因子：
3.1
作者：
Alice, Alejandro F.;Naka, Hiroaki;Crosa, Jorge H.
通讯作者：
Crosa, Jorge H.
影响因子：
3.1
作者：
Hofreuter, Dirk;Tsai, Jennifer;Galan, Jorge E.
通讯作者：
Galan, Jorge E.
影响因子：
3.2
作者：
Ridley, Kristian A.;Rock, Jonathan D.;Ketley, Julian M.
通讯作者：
Ketley, Julian M.