The evolution and adaptive potential of transcriptional variation in sticklebacks--signatures of selection and widespread heritability.
The evolution and adaptive potential of transcriptional variation in sticklebacks--signatures of selection and widespread heritability.
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DOI：
10.1093/molbev/msu328
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发表时间：
2015-03
影响因子：
10.7
通讯作者：
Merilä J
中科院分区：
文献类型：
--
作者：
Leder EH;McCairns RJ;Leinonen T;Cano JM;Viitaniemi HM;Nikinmaa M;Primmer CR;Merilä J
证据暗示差异基因表达作为进化新奇的重要驱动力继续积累，但我们对表达变异的潜在来源的理解，无论是环境还是遗传，都是不够的。特别是遗传可能被低估时，从遗传图谱研究，主要的“遗传基因组学”的方法来研究表达变异的推断。这种不确定性代表了在转录组水平上测试适应性进化的基本限制。通过研究由563个个体组成的三刺鱼谱系中10，495个基因（10，527个剪接变体）的表达水平的遗传，其中一半受到热处理，我们发现74-98%的转录本表现出显着的加性遗传变异。显性变异也很普遍（占转录本的41-99%），并且变异的遗传来源似乎比关键的环境变量温度在肝脏中的表达变异中发挥更重要的作用。群体间比较表明，大多数差异表达在肝脏中可能是由于中性分歧，但是，我们也表明，签名的定向选择可能比那些稳定的选择更普遍。这主要与基因表达的中性进化模型相一致，但也表明自然选择可能仍然作用于野生环境中的转录变异。由于群体内和群体间的遗传变异最终决定了适应潜力，这些结果表明，转录组内可能存在广泛的适应潜力。
Evidence implicating differential gene expression as a significant driver of evolutionary novelty continues to accumulate, but our understanding of the underlying sources of variation in expression, both environmental and genetic, is wanting. Heritability in particular may be underestimated when inferred from genetic mapping studies, the predominant “genetical genomics” approach to the study of expression variation. Such uncertainty represents a fundamental limitation to testing for adaptive evolution at the transcriptomic level. By studying the inheritance of expression levels in 10,495 genes (10,527 splice variants) in a threespine stickleback pedigree consisting of 563 individuals, half of which were subjected to a thermal treatment, we show that 74–98% of transcripts exhibit significant additive genetic variance. Dominance variance is also prevalent (41–99% of transcripts), and genetic sources of variation seem to play a more significant role in expression variance in the liver than a key environmental variable, temperature. Among-population comparisons suggest that the majority of differential expression in the liver is likely due to neutral divergence; however, we also show that signatures of directional selection may be more prevalent than those of stabilizing selection. This predominantly aligns with the neutral model of evolution for gene expression but also suggests that natural selection may still act on transcriptional variation in the wild. As genetic variation both within- and among-populations ultimately defines adaptive potential, these results indicate that broad adaptive potential may be found within the transcriptome.
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影响因子：
4.9
作者：
Cano, J. M.;Matsuba, C.;Merila, J.
通讯作者：
Merila, J.
影响因子：
4.5
作者：
Ghazalpour A;Bennett B;Petyuk VA;Orozco L;Hagopian R;Mungrue IN;Farber CR;Sinsheimer J;Kang HM;Furlotte N;Park CC;Wen PZ;Brewer H;Weitz K;Camp DG 2nd;Pan C;Yordanova R;Neuhaus I;Tilford C;Siemers N;Gargalovic P;Eskin E;Kirchgessner T;Smith DJ;Smith RD;Lusis AJ
通讯作者：
Lusis AJ
影响因子：
7
作者：
Emerson, J. J.;Hsieh, Li-Ching;Li, Wen-Hsiung
通讯作者：
Li, Wen-Hsiung
影响因子：
4.9
作者：
Debes, Paul V.;Normandeau, Eric;Hutchings, Jeffrey A.
通讯作者：
Hutchings, Jeffrey A.
影响因子：
3.3
作者：
Cui, Xiangqin;Affourtit, Jason;Churchill, Gary A.
通讯作者：
Churchill, Gary A.