Mass Spectrometry Imaging of Fibroblasts: Promise and Challenge.
Mass Spectrometry Imaging of Fibroblasts: Promise and Challenge.
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成纤维细胞的质谱成像：前景与挑战
DOI：
10.1080/14789450.2021.1941893
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发表时间：
2021-06
影响因子：
3.4
通讯作者：
Drake R
中科院分区：
文献类型：
--
作者：
Angel PM;Rujchanarong D;Pippin S;Spruill L;Drake R
成纤维细胞通过输出细胞外基质来维持组织和器官的内稳态，这种细胞外基质会影响基质内附近细胞的信号传导。成纤维细胞信号传导的改变会导致许多疾病状态，并且成纤维细胞分泌的细胞外基质已被用于根据结果、复发和治疗抗性对患者进行分层。成像质谱技术的最新进展使得在临床相关组织样本中能够获取单细胞成纤维细胞及其细胞外基质微环境。
我们综述了在复杂组织微环境中获取成纤维细胞蛋白质组表达所面临的生物学和技术挑战以及新的解决方案。综述主题涵盖了用于单细胞成纤维细胞分析的常规蛋白质组学方法，以及通过成像质谱方法获取单细胞成纤维细胞蛋白质组的当前方法。还介绍了基于细胞信号传导来靶向和评估单细胞基质蛋白质组的策略。
从成纤维细胞及其细胞外基质微环境中确定蛋白质组特征的前景在于发现新的疾病标志物以及改进治疗方法的能力。有几种成像质谱方法可用于从临床获取的样本中确定病理变化情况下的成纤维细胞。需要持续的技术进步来获取和理解基质蛋白质组，并将检测应用于临床。
Fibroblasts maintain tissue and organ homeostasis through output of extracellular matrix that affects nearby cell signaling within the stroma. Altered fibroblast signaling contributes to many disease states and the extracellular matrix secreted by fibroblasts has been used to stratify patient by outcome, recurrence and therapeutic resistance. Recent advances in imaging mass spectrometry allow access to single cell fibroblasts and their ECM niche within clinically relevant tissue samples. We review biological and technical challenges as well as new solutions to proteomic access of fibroblast expression within the complex tissue microenvironment. Review topics cover conventional proteomic methods for single fibroblast analysis and current approaches to accessing single fibroblast proteomes by imaging mass spectrometry approaches. Strategies to target and evaluate the single cell stroma proteome on the basis of cell signaling are presented. The promise of defining proteomic signatures from fibroblasts and their extracellular matrix niches is the discovery of new disease markers and the ability to refine therapeutic treatments. Several imaging mass spectrometry approaches exist to define the fibroblast in the setting of pathological changes from clinically acquired samples. Continued technology advances are needed to access and understand the stromal proteome and apply testing to the clinic.
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DOI：
10.1007/s13361-015-1143-9
发表时间：
2015-06-01
影响因子：
3.2
作者：
Anderson, David M. G.;Spraggins, Jeffrey M.;Schey, Kevin L.
通讯作者：
Schey, Kevin L.
DOI：
10.1007/s13361-015-1324-6
发表时间：
2016-03-01
影响因子：
3.2
作者：
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通讯作者：
Pasa-Tolic, Ljiljana
影响因子：
7.4
作者：
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通讯作者：
Caprioli, Richard
影响因子：
5
作者：
Angel PM;Drake RR;Park Y;Clift CL;West C;Berkhiser S;Hardiman G;Mehta AS;Bichell DP;Su YR
通讯作者：
Su YR
影响因子：
5.8
作者：
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通讯作者：
Maass, Peter