Enhanced sensitivity for high spatial resolution lipid analysis by negative ion mode matrix assisted laser desorption ionization imaging mass spectrometry.
Enhanced sensitivity for high spatial resolution lipid analysis by negative ion mode matrix assisted laser desorption ionization imaging mass spectrometry.
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DOI：
10.1021/ac202383m
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发表时间：
2012-02-07
影响因子：
7.4
通讯作者：
Caprioli, Richard
中科院分区：
文献类型：
--
作者：
Angel, Peggi M.;Spraggins, Jeffrey M.;Baldwin, H. Scott;Caprioli, Richard
我们利用负离子模式基质辅助激光解吸电离成像质谱学，以2，5-二羟基苯甲酸为基质升华，并采用水洗的样品制备方法，实现了对脂质成像的增强，空间分辨率达到10μm。我们报告了在负离子模式脂肪成像之前，用不同pH值的挥发性缓冲液清洗组织切片的效果。结果表明，用pH 6.4的甲酸铵或pH 6.7的醋酸铵洗涤，显著增加成年小鼠脑组织切片的信号强度和分析物的数量。测得的主要脂质种类为甘油磷酸肌醇、甘油磷酸酯、甘油磷甘油、甘油磷乙醇胺、甘油磷丝氨酸、硫脂和神经节苷脂。来自成年小鼠大脑切片的离子图像，比较了清洗和未清洗的切片，显示清洗组织的离子强度增加了五倍。样品制备方法已被发现适用于许多器官类型，并显著扩大了高空间分辨率成像质谱仪可检测到的类脂种类的数量。
We have achieved enhanced lipid imaging to a ~10 μm spatial resolution using negative ion mode matrix assisted laser desorption ionization (MALDI) imaging mass spectrometry, sublimation of 2,5-dihydroxybenzoic acid as the MALDI matrix and a sample preparation protocol that uses aqueous washes. We report on the effect of treating tissue sections by washing with volatile buffers at different pHs prior to negative ion mode lipid imaging. The results show that washing with ammonium formate, pH 6.4, or ammonium acetate, pH 6.7, significantly increases signal intensity and number of analytes recorded from adult mouse brain tissue sections. Major lipid species measured were glycerophosphoinositols, glycerophosphates, glycerolphosphoglycerols, glycerophosphoethanolamines, glycerophospho-serines, sulfatides, and gangliosides. Ion images from adult mouse brain sections that compare washed and unwashed sections are presented and show up to fivefold increases in ion intensity for washed tissue. The sample preparation protocol has been found to be applicable across numerous organ types and significantly expands the number of lipid species detectable by imaging mass spectrometry at high spatial resolution.
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影响因子：
7
作者：
Chaurand, Pierre;Cornett, Dale S.;Caprioli, Richard M.
通讯作者：
Caprioli, Richard M.
影响因子：
14.8
作者：
Brown, H. Alex;Murphy, Robert C.
通讯作者：
Murphy, Robert C.
影响因子：
6.5
作者：
Murphy, Robert C.;Hankin, Joseph A.;Barkley, Robert M.
通讯作者：
Barkley, Robert M.
影响因子：
2.9
作者：
Schiller, J;Süss, R;Arnold, K
通讯作者：
Arnold, K
DOI：
10.1016/s1388-1981(99)00201-2
发表时间：
2000-02-24
影响因子：
4.8
作者：
Marbois, BN;Faull, KF;Rome, LH
通讯作者：
Rome, LH