Mutation of 4-coumarate: coenzyme A ligase 1 gene affects lignin biosynthesis and increases the cell wall digestibility in maize brown midrib5 mutants
Mutation of 4-coumarate: coenzyme A ligase 1 gene affects lignin biosynthesis and increases the cell wall digestibility in maize brown midrib5 mutants
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4-香豆酸突变：辅酶 A 连接酶 1 基因影响木质素生物合成并增加玉米棕色中脉5突变体的细胞壁消化率
DOI：
10.1186/s13068-019-1421-z
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发表时间：
2019-04
影响因子：
6.3
通讯作者：
Fu Chunxiang
中科院分区：
文献类型：
--
作者：
Xiong Wangdan;Wu Zhenying;Liu Yuchen;Li Yu;Su Kunlong;Bai Zetao;Guo Siyi;Hu Zhubing;Zhang Zhiming;Bao Yan;Sun Juan;Yang Guofeng;Fu Chunxiang
背景：玉米褐中脉（bm）突变体与木质素生物合成受损相关，是培育具有改善细胞壁消化率的新型胚芽的潜在来源。自2008年以来，已经鉴定了自发bm 5突变体。结果：通过MutMap辅助的基因定位分析，确定bm 5基因编码一个主要的4-香豆酸：辅酶A连接酶（Zm 4CL 1）。从mbm 5突变体中分离到的Zm 4CL 1的两个等位基因分别在第一外显子和第二内含子中插入了两个转座子，因此mbm 5中脉粗酶提取物中4CLs的活性比野生型降低了51-62%。此外，从玉米基因组中检索到5个4CL，其中Zm 4CL 1在木质部组织中的表达量最高。Zm 4CL 1突变主要阻碍愈创木基木质素的生物合成，并增加可溶性阿魏酸衍生物的水平，而不影响玉米的生长发育。结论：Zm 4CL 1基因的两个独立等位基因与相同的突变表型相关，因此Zm 4CL 1为Bm 5基因。Zm 4CL 1基因突变主要影响玉米木质素组织中G木质素的合成和可溶性阿魏酸衍生物的积累。bm 5突变体的抗突变性降低表明Zm 4CL 1是细胞壁工程的一个优良靶基因，对该基因的遗传操作将有助于对环境保护中必须处理的农作物秸秆的利用。
Background:Maizebrown midrib(bm) mutants associated with impaired lignin biosynthesis are a potential source for the breed of novel germplasms with improved cell wall digestibility. The spontaneousbm5mutants had been identified since 2008. However, the gene responsible for thebm5locus, and the comprehensive effects ofbm5mutation on lignin biosynthesis, soluble phenolics accumulation, and cell wall degradation have yet to be elucidated.Results:Thebm5locus was identified to encode a major 4-coumarate: coenzyme A ligase (Zm4CL1) through analyzing MutMap-assisted gene mapping data. Two alleles ofZm4CL1isolated frombm5mutants contained two transposons inserted in the first exon and the second intron, respectively, and consequently, the activities of 4CLs in the crude enzyme extracts frombm5midribs were reduced by 51-62% compared with the wild type. Furthermore, five4CLswere retrieved from maize genome, andZm4CL1was the most highly expressed one in the lignified tissues. Mutation ofZm4CL1mainly impeded the biosynthesis of guaiacyl (G) lignins and increased the level of soluble feruloyl derivatives without impacting maize growth and development. Moreover, both neutral detergent fiber digestibility and saccharification efficiency of cell walls were significantly elevated in thebm5mutant.Conclusions:Zm4CL1 was identified as theBm5gene, since two independent alleles ofZm4CL1were associated with the same mutant phenotype. Mutation ofZm4CL1mainly affected G lignin biosynthesis and soluble feruloyl derivatives accumulation in maize lignified tissues. The reduced recalcitrance of thebm5mutant suggests thatZm4CL1is an elite target for cell wall engineering, and genetic manipulation of this gene will facilitate the utilization of crop straw and stover that have to be dealt with for environmental protection.
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DOI：
10.1111/tpj.12394
发表时间：
2014-02
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Tang HM;Liu S;Hill-Skinner S;Wu W;Reed D;Yeh CT;Nettleton D;Schnable PS
通讯作者：
Schnable PS
影响因子：
13.8
作者：
Fu C;Sunkar R;Zhou C;Shen H;Zhang JY;Matts J;Wolf J;Mann DG;Stewart CN Jr;Tang Y;Wang ZY
通讯作者：
Wang ZY
DOI：
--
发表时间：
1986-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
R. Villegas;M. Kojima
通讯作者：
R. Villegas;M. Kojima
影响因子：
5.6
作者：
Liu T;Yao R;Zhao Y;Xu S;Huang C;Luo J;Kong L
通讯作者：
Kong L
影响因子：
--
作者：
A. Rose
通讯作者：
A. Rose