Overexpression of miR156 in switchgrass (Panicum virgatum L.) results in various morphological alterations and leads to improved biomass production.
Overexpression of miR156 in switchgrass (Panicum virgatum L.) results in various morphological alterations and leads to improved biomass production.
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DOI：
10.1111/j.1467-7652.2011.00677.x
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发表时间：
2012-05
影响因子：
13.8
通讯作者：
Wang ZY
中科院分区：
文献类型：
--
作者：
Fu C;Sunkar R;Zhou C;Shen H;Zhang JY;Matts J;Wolf J;Mann DG;Stewart CN Jr;Tang Y;Wang ZY
柳枝稷（Panicum virgatum L.）已发展成为一种专用草本生物能源作物。生物量产量是柳枝稷遗传改良的主要目标性状。 microRNA 已成为一类重要的基因调控因子，有可能改善生物量产量等复杂性状。 miR156b 前体在柳枝稷中过度表达。通过微阵列和定量 RT-PCR 分析揭示了 miR156 过表达对 SQUAMOSA 启动子结合蛋白样 (SPL) 基因的影响。对转基因植物的形态变化、生物量产量、糖化效率和饲料消化率进行了表征。 miR156 通过抑制其目标 SPL 基因来控制柳枝稷的顶端优势和花转变。相对较低水平的 miR156 过表达足以增加生物量产量，同时产生具有正常开花时间的植物。中等水平的 miR156 导致生物量提高，但植物不开花。与对照组相比，这两组植物的生物量产量增加了 58%–101%。然而，高 miR156 水平导致生长严重迟缓。转基因柳枝稷形态改变的程度取决于 miR156 水平。与花转变相比，破坏顶端优势需要较低的 miR156 水平。生物量产量的提高主要是由于分蘖数的增加。 miR156 的靶向过度表达还提高了可溶糖产量和饲料消化率，并为转基因遏制提供了有效的方法。
Switchgrass (Panicum virgatum L.) has been developed into a dedicated herbaceous bioenergy crop. Biomass yield is a major target trait for genetic improvement of switchgrass. microRNAs have emerged as a prominent class of gene regulatory factors that has the potential to improve complex traits such as biomass yield. A miR156b precursor was overexpressed in switchgrass. The effects of miR156 overexpression on SQUAMOSA PROMOTER BINDING PROTEIN LIKE (SPL) genes were revealed by microarray and quantitative RT-PCR analyses. Morphological alterations, biomass yield, saccharification efficiency and forage digestibility of the transgenic plants were characterized. miR156 controls apical dominance and floral transition in switchgrass by suppressing its target SPL genes. Relatively low levels of miR156 overexpression were sufficient to increase biomass yield while producing plants with normal flowering time. Moderate levels of miR156 led to improved biomass but the plants were non-flowering. These two groups of plants produced 58%–101% more biomass yield compared with the control. However, high miR156 levels resulted in severely stunted growth. The degree of morphological alterations of the transgenic switchgrass depends on miR156 level. Compared with floral transition, a lower miR156 level is required to disrupt apical dominance. The improvement in biomass yield was mainly because of the increase in tiller number. Targeted overexpression of miR156 also improved solubilized sugar yield and forage digestibility, and offered an effective approach for transgene containment.
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