LncRNA MEG3-TRPV1 signaling regulates chronic inflammatory pain in rats.
LncRNA MEG3-TRPV1 signaling regulates chronic inflammatory pain in rats.
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LncRNA MEG3-TRPV1信号调节大鼠慢性炎症疼痛
DOI：
10.1177/17448069221144246
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发表时间：
2022-04
期刊：
影响因子：
3.3
通讯作者：
Zhang, Hai-Long
中科院分区：
文献类型：
--
作者：
Peng, Jing-Wei;Gu, Yin-Yin;Wei, Jia;Sun, Ye;Zhu, Chun-Long;Zhang, Ling;Song, Yu;Chen, Long;Chen, Xia;Wang, Qian;Zhang, Hai-Long
骨关节炎是中老年人常见的一种以慢性炎症性疼痛为核心症状的骨关节病。LncRNA MEG3(母系表达基因3)通过调节血管生成参与骨性关节炎的发生发展，导致瞬时受体电位香草样物质-1(TRPV1)的激活和过度表达。本研究旨在探讨MEG3-TRPV1信号在慢性炎症性疼痛(CIP)大鼠模型中的作用机制。大鼠左后爪皮下注射完全弗氏佐剂(CFA)复制慢性炎症性疼痛模型。结果表明，在CFA诱导的大鼠背根神经节和SDH中，TRPV1的mRNA和蛋白显著增加，而MEG3的mRNA显著减少。此外，鞘内注射MEG3高表达慢病毒可显著下调CFA诱导的大鼠TRPV1的表达，减轻慢性炎性疼痛。TRPV1拮抗剂的治疗也显著缓解了CFA诱导的大鼠的慢性炎性疼痛。总体而言，我们的结果显示MEG3通过下调TRPV1的表达来缓解慢性炎症性疼痛。这些发现可能为骨性关节炎患者的治疗提供新的治疗靶点。
Osteoarthritis (OA) is a common osteoarthropathy with chronic inflammatory pain as the core symptom in middle-aged and elderly people. LncRNA MEG3 (Maternally expressed gene 3) is involved in the development of OA via regulation of angiogenesis, which causes the activation and overexpression of transient receptor potential vanilloid type-1 (TRPV1). In this study, we investigated the mechanism of MEG3-TRPV1 signaling in chronic inflammatory pain (CIP) of rat model. Chronic inflammatory pain was modeled using subcutaneous microinjection of complete Freund’s adjuvant (CFA) into the left hind paw of rats. We showed that TRPV1 mRNA and protein were significantly increased, while MEG3 mRNA was significantly decreased, in the DRG and SDH of CFA-induced rats. In addition, intrathecal injection of MEG3-overexpressing lentivirus significantly downregulated TRPV1 expression and alleviated chronic inflammatory pain in CFA-induced rats. Treatment with a TRPV1 antagonist also significantly relieved chronic inflammatory pain in CFA-induced rats. In general, our results reveal that MEG3 alleviates chronic inflammatory pain by downregulating TRPV1 expression. These findings may provide new therapeutic targets in the treatment of patients with OA.
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影响因子：
3.3
作者：
Lu JS;Chen QY;Chen X;Li XH;Zhou Z;Liu Q;Lin Y;Zhou M;Xu PY;Zhuo M
通讯作者：
Zhuo M
影响因子：
168.9
作者：
Glyn-Jones, S.;Palmer, A. J. R.;Carr, A. J.
通讯作者：
Carr, A. J.
影响因子：
2.8
作者：
Chang, Chih-Hung;Hsu, Yuan-Ming;Savitha, Sivasubramanian
通讯作者：
Savitha, Sivasubramanian
影响因子：
2.1
作者：
Miyoshi, N;Wagatsuma, H;Ishino, F
通讯作者：
Ishino, F
影响因子：
5.4
作者：
Lee, Sung-Gyu;Lee, Eun-Ju;Choi, Sang-Won
通讯作者：
Choi, Sang-Won