Dissolving Microneedle Delivery of a Prophylactic HPV Vaccine.
Dissolving Microneedle Delivery of a Prophylactic HPV Vaccine.
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DOI：
10.1021/acs.biomac.1c01345
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发表时间：
2022-03-14
影响因子：
6.2
通讯作者：
Pokorski, Jonathan K.
中科院分区：
文献类型：
--
作者：
Ray, Sayoni;Wirth, David M.;Ortega-Rivera, Oscar A.;Steinmetz, Nicole F.;Pokorski, Jonathan K.
由于成本高昂以及在医疗环境中进行多次接种所面临的挑战，全球绝大多数人口仍然无法获得能够预防人乳头瘤病毒 (HPV) 感染的预防性疫苗。为了改善分发和管理，我们开发了可溶解的微针，装载有热稳定的 HPV 候选疫苗，该候选疫苗由 Qβ 病毒样颗粒 (VLP) 组成，显示 HPV L2 蛋白 (Qβ-HPV) 高度保守的表位。与传统皮下注射相比，Qβ-HPV 的聚合物微针递送产生相似数量的抗 HPV16 L2 IgG 抗体，同时递送的皮内剂量要少得多。然而，发现了剂量节约效应。此外，在 HPV 假病毒检测中，免疫产生了中和抗体反应。该候选疫苗在储存几个月后被证实在室温下保持稳定，可能缓解与冷链分销相关的许多挑战。此类微针贴片疫苗易于自我给药且侵入性极小，可能有助于 HPV 疫苗的大规模分发，并提高患者的依从性。 Qβ VLP 及其交付技术是一种即插即用系统，可以作为具有广泛应用的通用平台。 Qβ VLP 可以储存用于与各种表位缀合，然后将其包装并通过非侵入性微针贴片直接递送给患者。这样的系统为疫苗的快速分发和自我管理铺平了道路。
Prophylactic vaccines capable of preventing human papillomavirus (HPV) infections are still inaccessible to a vast majority of the global population due to their high cost and challenges related to multiple administrations performed in a medical setting. In an effort to improve distribution and administration, we have developed dissolvable microneedles loaded with a thermally stable HPV vaccine candidate consisting of Qβ virus-like particles (VLPs) displaying a highly conserved epitope from the L2 protein of HPV (Qβ-HPV). Polymeric microneedle delivery of Qβ-HPV produces similar amounts of anti-HPV16 L2 IgG antibodies compared to traditional subcutaneous injection while delivering a much smaller amount of intradermal dose. However, a dose sparing effect was found. Furthermore, immunization yielded neutralizing antibody responses in a HPV pseudovirus assay. The vaccine candidate was confirmed to be stable at room temperature after storage for several months, potentially mitigating many of the challenges associated with cold-chain distribution. The ease of self-administration and minimal invasiveness of such microneedle patch vaccines may enable wide-scale distribution of the HPV vaccine and lead to higher patient compliance. The Qβ VLP and its delivery technology is a plug-and-play system that could serve as a universal platform with a broad range of applications. Qβ VLPs may be stockpiled for conjugation to a wide range of epitopes, which are then packaged and delivered directly to the patient via noninvasive microneedle patches. Such a system paves the way for rapid distribution and self-administration of vaccines.
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影响因子：
15
作者：
Prasuhn, Duane E., Jr.;Singh, Pratik;Finn, M. G.
通讯作者：
Finn, M. G.
DOI：
10.15585/mmwr.mm6429a3
发表时间：
2015-07-31
期刊：
MMWR. Morbidity and mortality weekly report
影响因子：
--
作者：
Reagan-Steiner S;Yankey D;Jeyarajah J;Elam-Evans LD;Singleton JA;Curtis CR;MacNeil J;Markowitz LE;Stokley S
通讯作者：
Stokley S
影响因子：
7.8
作者：
Shao S;A Ortega-Rivera O;Ray S;K Pokorski J;F Steinmetz N
通讯作者：
F Steinmetz N
影响因子：
--
作者：
Day, Patricia M.;Pang, Yuk-Ying S.;Schiller, John T.
通讯作者：
Schiller, John T.
影响因子：
4.9
作者：
Pokorski JK;Steinmetz NF
通讯作者：
Steinmetz NF