Single-cell intracellular nano-pH probes.
Single-cell intracellular nano-pH probes.
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DOI：
10.1039/c5ra06721a
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发表时间：
2015
期刊：
影响因子：
3.9
通讯作者：
Pourmand N
中科院分区：
文献类型：
--
作者：
Özel RE;Lohith A;Mak WH;Pourmand N
在一个大的克隆群体中，如癌性肿瘤实体，细胞是不相同的，单个细胞的细胞内pH水平之间的差异可能是异质性的重要指标，这可能与临床实践有关，特别是在个性化医疗中。因此，在单细胞水平上检测细胞内pH值对于识别和研究异常细胞具有重要意义。然而，定量和实时测量细胞群内单个细胞的细胞内pH值对现有技术具有挑战性，并且需要设计新的方法。在本文中，我们讨论了使用纳米移液管技术，以克服在单细胞水平的细胞内pH值测量的局限性。我们已经开发了一种纳米pH探针，通过物理吸附壳聚糖到羟基化的石英纳米移液管具有非常小的孔径（~100 nm）。纳米pH探针的动态pH范围为2.6至10.7，灵敏度为0.09单位。我们使用非癌和癌细胞系，包括人成纤维细胞、HeLa、MDA-MB-231和MCF-7，用pH纳米探针进行了单细胞细胞内pH测量。我们进一步证明了传感器的实时连续单细胞pH测量能力，显示了细胞pH对药物操作的响应。这些研究结果表明，壳聚糖功能化的纳米孔是一个强大的纳米工具，在单细胞水平的pH传感具有高的时间和空间分辨率。
Within a large clonal population, such as cancerous tumor entities, cells are not identical, and the differences between intracellular pH levels of individual cells may be important indicators of heterogeneity that could be relevant in clinical practice, especially in personalized medicine. Therefore, the detection of the intracellular pH at the single-cell level is of great importance to identify and study outlier cells. However, quantitative and real-time measurements of the intracellular pH of individual cells within a cell population is challenging with existing technologies, and there is a need to engineer new methodologies. In this paper, we discuss the use of nanopipette technology to overcome the limitations of intracellular pH measurements at the single-cell level. We have developed a nano-pH probe through physisorption of chitosan onto hydroxylated quartz nanopipettes with extremely small pore sizes (~100 nm). The dynamic pH range of the nano-pH probe was from 2.6 to 10.7 with a sensitivity of 0.09 units. We have performed single-cell intracellular pH measurements using non-cancerous and cancerous cell lines, including human fibroblasts, HeLa, MDA-MB-231 and MCF-7, with the pH nanoprobe. We have further demonstrated the real-time continuous single-cell pH measurement capability of the sensor, showing the cellular pH response to pharmaceutical manipulations. These findings suggest that the chitosan-functionalized nanopore is a powerful nano-tool for pH sensing at the single-cell level with high temporal and spatial resolution.
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壳聚糖刷的对称pH依赖性肿胀和抗菌特性。
DOI：
10.1021/la202616u
发表时间：
2011-10-18
期刊：
Langmuir : the ACS journal of surfaces and colloids
影响因子：
--
作者：
Lee HS;Eckmann DM;Lee D;Hickok NJ;Composto RJ
通讯作者：
Composto RJ
DOI：
10.1021/la2005612
发表时间：
2011-05-17
期刊：
Langmuir : the ACS journal of surfaces and colloids
影响因子：
--
作者：
Actis P;Vilozny B;Seger RA;Li X;Jejelowo O;Rinaudo M;Pourmand N
通讯作者：
Pourmand N
影响因子：
2.9
作者：
Hashim, Arig Ibrahim;Zhang, Xiaomeng;Wojtkowiak, Jonathan W.;Martinez, Gary V.;Gillies, Robert J.
通讯作者：
Gillies, Robert J.
影响因子：
3.9
作者：
CLAESSON, PM;NINHAM, BW
通讯作者：
NINHAM, BW
影响因子：
7.3
作者：
Brown, CDA;Dudley, AJ
通讯作者：
Dudley, AJ