Design of a multi-epitope vaccine against brucellosis fused to IgG-fc by an immunoinformatics approach.
Design of a multi-epitope vaccine against brucellosis fused to IgG-fc by an immunoinformatics approach.
复制标题
DOI：
10.3389/fvets.2023.1238634
复制
发表时间：
2023
影响因子：
3.2
通讯作者：
中科院分区：
文献类型：
--
作者：
布鲁氏菌是一种细胞内革兰氏阴性菌，具有独特的特征，是一种人畜共患病原体。它会导致动物流产和不育。一旦布鲁氏菌病在人类和动物之间传播，消除它就变得非常具有挑战性，给全世界的牲畜和人带来沉重负担。鉴于布鲁氏菌病的日益蔓延，为易感动物开发改进的疫苗以减少疾病的影响至关重要。在这项研究中，我们有效地使用免疫信息学方法和先进的计算机软件来仔细鉴定和分析流产布鲁氏菌的重要抗原部分。随后，我们巧妙地设计了嵌合肽，以增强疫苗的强度和有效性。我们用电脑程序找到了免疫系统能识别的布鲁氏菌的四个重要部分。然后，我们仔细地寻找了被一种叫做细胞毒性T细胞的白细胞识别的8个部分，T辅助细胞识别的6个部分，B细胞识别的4个部分。我们用一种特殊的连接将这些部分连接在一起，制造出一种强效的新疫苗。为了使疫苗更好，我们添加了一些叫做分子佐剂的额外部分。其中包括我们在数据库中发现的人类β-防御素3 (hBD-3)，以及另一个帮助免疫系统的部分——PADRE。我们把这些额外的部分附加到疫苗的开头。通过一种新颖而聪明的方法，我们将老鼠免疫系统的一部分附加到疫苗的末端，从而使疫苗更加强大。这就产生了一种叫做MEV-Fc的新型疫苗。我们使用先进的计算机方法来研究MEV-Fc疫苗如何与体内某些受体（TLR-2和TLR-4）相互作用。最后，免疫模拟预测表明，MEV-Fc疫苗可以使免疫系统在细胞和抗体方面产生强烈反应。总之，我们的结果为布鲁氏菌疫苗的开发提供了新的见解。虽然需要进一步的实验室实验来评估其保护作用。
Brucella, a type of intracellular Gram-negative bacterium, has unique features and acts as a zoonotic pathogen. It can lead to abortion and infertility in animals. Eliminating brucellosis becomes very challenging once it spreads among both humans and animals, putting a heavy burden on livestock and people worldwide. Given the increasing spread of brucellosis, it is crucial to develop improved vaccines for susceptible animals to reduce the disease’s impact. In this study, we effectively used an immunoinformatics approach with advanced computer software to carefully identify and analyze important antigenic parts of Brucella abortus. Subsequently, we skillfully designed chimeric peptides to enhance the vaccine’s strength and effectiveness. We used computer programs to find four important parts of the Brucella bacteria that our immune system recognizes. Then, we carefully looked for eight parts that are recognized by a type of white blood cell called cytotoxic T cells, six parts recognized by T helper cells, and four parts recognized by B cells. We connected these parts together using a special link, creating a strong new vaccine. To make the vaccine even better, we added some extra parts called molecular adjuvants. These included something called human β-defensins 3 (hBD-3) that we found in a database, and another part that helps the immune system called PADRE. We attached these extra parts to the beginning of the vaccine. In a new and clever way, we made the vaccine even stronger by attaching a part from a mouse’s immune system to the end of it. This created a new kind of vaccine called MEV-Fc. We used advanced computer methods to study how well the MEV-Fc vaccine interacts with certain receptors in the body (TLR-2 and TLR-4). In the end, Immunosimulation predictions showed that the MEV-Fc vaccine can make the immune system respond strongly, both in terms of cells and antibodies. In summary, our results provide novel insights for the development of Brucella vaccines. Although further laboratory experiments are required to assess its protective effect.
登录
查看更多内容
影响因子：
8
作者：
COLOVOS, C;YEATES, TO
通讯作者：
YEATES, TO
影响因子：
4.4
作者：
Dorneles EM;Sriranganathan N;Lage AP
通讯作者：
Lage AP
影响因子：
3.1
作者：
Barrionuevo, Paula;Cassataro, Juliana;Giambartolomei, Guillermo H.
通讯作者：
Giambartolomei, Guillermo H.
影响因子：
2.6
作者：
Godfroid, J.;Scholz, H. C.;Letesson, J. -J.
通讯作者：
Letesson, J. -J.
影响因子：
3.6
作者：
Goel, Divya;Bhatnagar, Rakesh
通讯作者：
Bhatnagar, Rakesh