A role for actin, Cdc1p, and Myo2p in the inheritance of late Golgi elements in Saccharomyces cerevisiae.
A role for actin, Cdc1p, and Myo2p in the inheritance of late Golgi elements in Saccharomyces cerevisiae.
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DOI：
10.1083/jcb.153.1.47
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发表时间：
2001-04-02
期刊：
影响因子：
--
通讯作者：
Glick BS
中科院分区：
文献类型：
--
作者：
Rossanese OW;Reinke CA;Bevis BJ;Hammond AT;Sears IB;O'Connor J;Glick BS
在酿酒酵母中，高尔基体元素在细胞周期的早期就存在于芽中。我们用荧光显微镜和遗传学分析了这个高尔基遗传过程。在快速生长的细胞中，晚期高尔基元件在极化生长部位显示出肌动蛋白依赖的浓度。晚期高尔基体元素显然是沿着肌动蛋白索运输到芽中，并通过一种可能与肌动蛋白有关的机制保留在芽中。对晚期高尔基元素遗传缺陷突变体的视觉筛选产生了CDC1的多个等位基因。CDC1突变严重地使肌动蛋白细胞骨架去极化，这些突变阻止晚期高尔基体元素保留在芽中。晚期高尔基体元件有效定位到芽需要V型肌球蛋白Myo2p，进一步表明肌动蛋白在高尔基体遗传中起作用。令人惊讶的是，早期和晚期高尔基体元件通过不同的途径遗传，早期高尔基体元件以Cdc1p-和myo2p -独立的方式定位于芽。我们认为早期的高尔基体元素起源于存在于芽中的内质网膜。酿酒酵母的这两条高尔基遗传途径类似于脊椎动物细胞的高尔基遗传途径。
In Saccharomyces cerevisiae, Golgi elements are present in the bud very early in the cell cycle. We have analyzed this Golgi inheritance process using fluorescence microscopy and genetics. In rapidly growing cells, late Golgi elements show an actin-dependent concentration at sites of polarized growth. Late Golgi elements are apparently transported into the bud along actin cables and are also retained in the bud by a mechanism that may involve actin. A visual screen for mutants defective in the inheritance of late Golgi elements yielded multiple alleles of CDC1. Mutations in CDC1 severely depolarize the actin cytoskeleton, and these mutations prevent late Golgi elements from being retained in the bud. The efficient localization of late Golgi elements to the bud requires the type V myosin Myo2p, further suggesting that actin plays a role in Golgi inheritance. Surprisingly, early and late Golgi elements are inherited by different pathways, with early Golgi elements localizing to the bud in a Cdc1p- and Myo2p-independent manner. We propose that early Golgi elements arise from ER membranes that are present in the bud. These two pathways of Golgi inheritance in S. cerevisiae resemble Golgi inheritance pathways in vertebrate cells.
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影响因子：
3.3
作者：
Hammond, AT;Glick, BS
通讯作者：
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