A chitin deacetylase-like protein is a predominant constituent of tick peritrophic membrane that influences the persistence of Lyme disease pathogens within the vector.
A chitin deacetylase-like protein is a predominant constituent of tick peritrophic membrane that influences the persistence of Lyme disease pathogens within the vector.
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DOI：
10.1371/journal.pone.0078376
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Pal U
中科院分区：
文献类型：
--
作者：
Kariu T;Smith A;Yang X;Pal U
肩胛硬蜱是许多全球流行感染的特定节肢动物载体，包括由伯氏疏螺旋体细菌引起的莱姆病。一种由摄食诱导的脱细胞上皮屏障，称为围食膜（PM），在I。肩胛肌然而，PM是否或如何影响主要蜱传病原体的持久性，如B。Burgdorferi，仍然在很大程度上未知。对从喂食蜱中分离的PM进行基于质谱的蛋白质组分析，结果显示膜含有少量可检测的蛋白质，包括与节肢动物几丁质脱乙酰酶（CDA）同源的主要和免疫原性的60 kDa蛋白质，本文称为I.肩胛肌CDA样蛋白或IsCDA。虽然IsCDA主要在肠道中表达，并在蜱虫进食早期诱导，但通过RNA干扰沉默IsCDA未能影响PM或螺旋体持续存在的发生，表明IsCDA在蜱虫生物学和宿主-病原体相互作用中的冗余作用。然而，用抗IsCDA（PM最主要的蛋白质组分之一）的抗体处理蜱，影响了B。Burgdorferi生存，显着增加蜱虫内病原体水平，但不影响肠道细菌总数水平。这些研究表明，蜱PM在限制B的持久性方面具有优先作用。burgdorferi在载体中。进一步了解媒介成分有助于病原体存活的机制可能有助于开发新的策略来干扰感染。
Ixodes scapularis is the specific arthropod vector for a number of globally prevalent infections, including Lyme disease caused by the bacterium Borrelia burgdorferi. A feeding-induced and acellular epithelial barrier, known as the peritrophic membrane (PM) is detectable in I. scapularis. However, whether or how the PM influences the persistence of major tick-borne pathogens, such as B. burgdorferi, remains largely unknown. Mass spectrometry-based proteome analyses of isolated PM from fed ticks revealed that the membrane contains a few detectable proteins, including a predominant and immunogenic 60 kDa protein with homology to arthropod chitin deacetylase (CDA), herein termed I. scapularis CDA-like protein or IsCDA. Although IsCDA is primarily expressed in the gut and induced early during tick feeding, its silencing via RNA interference failed to influence either the occurrence of the PM or spirochete persistence, suggesting a redundant role of IsCDA in tick biology and host-pathogen interaction. However, treatment of ticks with antibodies against IsCDA, one of the most predominant protein components of PM, affected B. burgdorferi survival, significantly augmenting pathogen levels within ticks but without influencing the levels of total gut bacteria. These studies suggested a preferential role of tick PM in limiting persistence of B. burgdorferi within the vector. Further understanding of the mechanisms by which vector components contribute to pathogen survival may help the development of new strategies to interfere with the infection.
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DOI：
10.1086/605846
发表时间：
2009-10-15
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Zhang X;Yang X;Kumar M;Pal U
通讯作者：
Pal U
影响因子：
3.6
作者：
Kariu T;Yang X;Marks CB;Zhang X;Pal U
通讯作者：
Pal U
影响因子：
5.4
作者：
Jakubowska, Agata K.;Caccia, Silvia;Herrero, Salvador
通讯作者：
Herrero, Salvador
影响因子：
3.8
作者：
Guo, W;Li, GX;Wang, P
通讯作者：
Wang, P
影响因子：
1.2
作者：
Kariu, Toru;Coleman, Adam S.;Pal, Utpal
通讯作者：
Pal, Utpal