BB0323 function is essential for Borrelia burgdorferi virulence and persistence through tick-rodent transmission cycle.
BB0323 function is essential for Borrelia burgdorferi virulence and persistence through tick-rodent transmission cycle.
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DOI：
10.1086/605846
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发表时间：
2009-10-15
期刊：
影响因子：
--
通讯作者：
Pal U
中科院分区：
文献类型：
--
作者：
Zhang X;Yang X;Kumar M;Pal U
伯氏疏螺旋体bb0323编码包括人类在内的哺乳动物宿主中的一种免疫原蛋白。对体内bb0323表达的分析表明，在螺旋体感染周期中，bb0323的转录是可变的，在壁虱-小鼠传播过程中表达上调。感染性伯氏杆菌中bb0323的缺失不影响微生物在体外的存活，尽管生长动力学和细胞形态发生了显著变化。Bb0323突变株既不能感染小鼠，也不能感染扁虱，并且在接种的最初几天内，很快就从免疫活性和免疫缺陷的宿主和载体中被消除。突变株与天然bb0323的染色体互补和体内表型分析表明，螺旋体的毒力显著恢复，并在整个感染周期中持续存在。BB0323可能发挥着不可或缺的生理功能，在体内微生物持续存在和宿主与载体之间的过渡过程中更加明显。干扰BB0323功能的策略可能会中断螺旋体的感染循环。
Borrelia burgdorferi bb0323 encodes an immunogenic protein in mammalian hosts including humans. An analysis of bb0323 expression in vivo showed variable transcription throughout the spirochete infection cycle, with elevated expression during tick-mouse transmission. Deletion of bb0323 in infectious B. burgdorferi did not affect microbial survival in vitro, despite significant alterations in growth kinetics and cell morphology. bb0323 mutants were unable to infect either mice or ticks, and were quickly eliminated from immunocompetent and immunodeficient hosts and the vector, within the first few days of inoculation. Chromosomal complementation of the mutant with native bb0323 and phenotypic analysis in vivo indicated the significant restoration of spirochete virulence and persistence throughout the mouse-tick infection cycle. BB0323 may serve an indispensable physiological function that is more pronounced during microbial persistence and transitions between the host and the vector in vivo. Strategies to interfere with BB0323 function may interrupt the infectious cycle of spirochetes.
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