Proteomic features of skeletal muscle adaptation to resistance exercise training as a function of age.
Proteomic features of skeletal muscle adaptation to resistance exercise training as a function of age.
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DOI：
10.1007/s11357-022-00658-5
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发表时间：
2023-06
期刊：
影响因子：
5.6
通讯作者：
Etheridge, Timothy
中科院分区：
文献类型：
--
作者：
Deane, Colleen S.;Phillips, Bethan E.;Willis, Craig R. G.;Wilkinson, Daniel J.;Smith, Ken;Higashitani, Nahoko;Williams, John P.;Szewczyk, Nathaniel J.;Atherton, Philip J.;Higashitani, Atsushi;Etheridge, Timothy
阻力运动训练（RET）可以抵消肌肉老化的负面特征，但年龄较大与RET的适应能力降低有关。改变的肌肉蛋白质网络可能有助于衰老RET适应;因此，相关的蛋白质组范围的反应值得探索。我们采用定量肌浆蛋白质组学比较年龄相关的蛋白质组和磷酸化蛋白质组反应RET。在20周监督RET之前和之后，从8名年轻（25 ± 1.1岁）和8名老年（67.5 ± 2.6岁）成人中收集大腿肌肉活检。将每种条件下的肌肉肌浆组分合并，并使用相对和绝对定量的等压标签（iTRAQ）标记、串联质谱和基于网络的中心蛋白鉴定进行分析。老年人在全身瘦体重、体脂百分比和大腿瘦体重方面表现出RET诱导的适应性受损（P > 0.05）。iTRAQ鉴定了73种随年龄和/或RET差异表达的蛋白质。尽管有可能的蛋白质组随机性，RET改善了线粒体功能和葡萄糖代谢的衰老特征（顶部枢纽; PYK（丙酮酸激酶）），但未能纠正细胞骨架蛋白的衰老表达改变（顶部枢纽; YWHAZ（14-3-3蛋白ζ/δ））。这些老化的RET蛋白质组概况一般不变或相反调节后，在年轻的肌肉RET。同样，RET纠正了10种磷蛋白在衰老过程中的表达变化，但这些反应与年轻人再次不同。老年肌肉的特点是RET诱导的代谢蛋白质谱，而不是在年轻的肌肉，改善未经训练的年龄相关的蛋白质组缺陷。结合受损的细胞骨架粘附反应，这些结果提供了一个蛋白质组学框架，了解和优化老化肌肉RET适应。在线版本包含补充材料，可通过10.1007/s11357-022-00658-5获得。
Resistance exercise training (RET) can counteract negative features of muscle ageing but older age associates with reduced adaptive capacity to RET. Altered muscle protein networks likely contribute to ageing RET adaptation; therefore, associated proteome-wide responses warrant exploration. We employed quantitative sarcoplasmic proteomics to compare age-related proteome and phosphoproteome responses to RET. Thigh muscle biopsies were collected from eight young (25 ± 1.1 years) and eight older (67.5 ± 2.6 years) adults before and after 20 weeks supervised RET. Muscle sarcoplasmic fractions were pooled for each condition and analysed using Isobaric Tags for Relative and Absolute Quantification (iTRAQ) labelling, tandem mass spectrometry and network-based hub protein identification. Older adults displayed impaired RET-induced adaptations in whole-body lean mass, body fat percentage and thigh lean mass (P > 0.05). iTRAQ identified 73 differentially expressed proteins with age and/or RET. Despite possible proteomic stochasticity, RET improved ageing profiles for mitochondrial function and glucose metabolism (top hub; PYK (pyruvate kinase)) but failed to correct altered ageing expression of cytoskeletal proteins (top hub; YWHAZ (14–3-3 protein zeta/delta)). These ageing RET proteomic profiles were generally unchanged or oppositely regulated post-RET in younger muscle. Similarly, RET corrected expression of 10 phosphoproteins altered in ageing, but these responses were again different vs. younger adults. Older muscle is characterised by RET-induced metabolic protein profiles that, whilst not present in younger muscle, improve untrained age-related proteomic deficits. Combined with impaired cytoskeletal adhesion responses, these results provide a proteomic framework for understanding and optimising ageing muscle RET adaptation. The online version contains supplementary material available at 10.1007/s11357-022-00658-5.
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DOI：
10.1113/jp272857
发表时间：
2016-12-15
期刊：
The Journal of physiology
影响因子：
--
作者：
Brook MS;Wilkinson DJ;Mitchell WK;Lund JN;Phillips BE;Szewczyk NJ;Greenhaff PL;Smith K;Atherton PJ
通讯作者：
Atherton PJ
年轻人和老年人废用性肌肉萎缩与抗阻运动引起的肥大的转录组荟萃分析。
DOI：
10.1002/jcsm.12706
发表时间：
2021-06
期刊：
Journal of cachexia, sarcopenia and muscle
影响因子：
--
作者：
Deane CS;Willis CRG;Phillips BE;Atherton PJ;Harries LW;Ames RM;Szewczyk NJ;Etheridge T
通讯作者：
Etheridge T
影响因子：
64.8
作者：
Herndon, LA;Schmeissner, PJ;Driscoll, M
通讯作者：
Driscoll, M
影响因子：
3.4
作者：
Holloway, Kathryn V.;O'Gorman, Martin;Burniston, Jatin G.
通讯作者：
Burniston, Jatin G.
DOI：
10.1093/gerona/glw109
发表时间：
2017-05-01
影响因子：
5.1
作者：
Hughes, David C.;Marcotte, George R.;Baar, Keith
通讯作者：
Baar, Keith