Rapid fluorescence lifetime estimation with modified phasor approach and Laguerre deconvolution: a comparative study.

Rapid fluorescence lifetime estimation with modified phasor approach and Laguerre deconvolution: a comparative study.
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DOI:
10.1088/2050-6120/aa7b62
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发表时间:
2017-09-01
影响因子:
3.2
通讯作者:
Marcu L
Marcu L
中科院分区:
化学3区
文献类型:
--
作者:
Fereidouni F;Gorpas D;Ma D;Fatakdawala H;Marcu L

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荧光寿命成像已被证明是一个有价值的工具,询问和诊断的生物组织在介观水平。分析荧光衰减曲线以实时提取寿命值的能力对于临床转化和应用(例如肿瘤边缘描绘或动脉粥样硬化斑块的冠状动脉内成像)至关重要。在这项工作中,我们比较了两种流行的非参数(无拟合)方法的性能,用于确定荧光衰减的实时寿命值-相量方法和Laguerre反卷积。我们展示了模拟和实验数据的结果,比较这两种方法的精度和速度,以及它们对噪声和模型参数的依赖性。
Fluorescence lifetime imaging has been shown to serve as a valuable tool for interrogating and diagnosis of biological tissue at a mesoscopic level. The ability to analyze fluorescence decay curves to extract lifetime values in real-time is crucial for clinical translation and applications such as tumor margin delineation or intracoronary imaging of atherosclerotic plaques. In this work, we compare the performance of two popular non-parametric (fit-free) methods for determining lifetime values from fluorescence decays in real-time –the Phasor approach and Laguerre deconvolution. We demonstrate results from simulated and experimental data to compare the accuracy and speed of both methods and their dependence on noise and model parameters.
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