Rapid fluorescence lifetime estimation with modified phasor approach and Laguerre deconvolution: a comparative study.
Rapid fluorescence lifetime estimation with modified phasor approach and Laguerre deconvolution: a comparative study.
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DOI:
10.1088/2050-6120/aa7b62
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发表时间:
2017-09-01
影响因子:
3.2
通讯作者:
Marcu L
中科院分区:
文献类型:
--
作者:
Fereidouni F;Gorpas D;Ma D;Fatakdawala H;Marcu L
Fluorescence lifetime imaging has been shown to serve as a valuable tool for interrogating and diagnosis of biological tissue at a mesoscopic level. The ability to analyze fluorescence decay curves to extract lifetime values in real-time is crucial for clinical translation and applications such as tumor margin delineation or intracoronary imaging of atherosclerotic plaques. In this work, we compare the performance of two popular non-parametric (fit-free) methods for determining lifetime values from fluorescence decays in real-time –the Phasor approach and Laguerre deconvolution. We demonstrate results from simulated and experimental data to compare the accuracy and speed of both methods and their dependence on noise and model parameters.
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