Tradeoff between lag time and growth rate drives the plasmid acquisition cost.
Tradeoff between lag time and growth rate drives the plasmid acquisition cost.
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DOI：
10.1038/s41467-023-38022-6
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发表时间：
2023-04-24
影响因子：
16.6
通讯作者：
Lopatkin, Allison J.
中科院分区：
文献类型：
--
作者：
Ahmad, Mehrose;Prensky, Hannah;Balestrieri, Jacqueline;ElNaggar, Shahd;Gomez-Simmonds, Angela;Uhlemann, Anne-Catrin;Traxler, Beth;Singh, Abhyudai;Lopatkin, Allison J.
共轭质粒驱动微生物种群的遗传多样性和进化。尽管普遍存在，但质粒可以对其宿主施加长期的适应性成本，改变种群结构，生长动力学和进化结果。除了长期的适应性成本外，获得新的质粒会给细胞带来立即的短期扰动。然而，由于这种质粒收购成本的瞬时性质，其生理表现，整体幅度和人口水平的影响，定量的了解，仍然不清楚。为了解决这个问题，我们在这里跟踪质粒获得后立即生长的单菌落。我们发现，质粒收购成本主要是由滞后时间的变化，而不是增长率，近60个条件，涵盖不同的质粒，选择环境，和临床菌株/物种。令人惊讶的是，对于一个昂贵的质粒，克隆表现出较长的滞后时间也实现了更快的恢复增长率，这表明进化的权衡。建模和实验表明，这种权衡导致违反直觉的生态动态，即中等成本的质粒胜过他们的低成本和高成本的同行。这些结果表明，与健身成本，质粒收购动态并不均匀驱动的最小化生长劣势。此外，滞后/生长权衡在预测进行接合的细菌的生态结果和干预策略方面具有明确的意义。质粒的获得对细菌宿主造成短暂的负担。在这里，作者表明，这种负担导致生长和滞后之间的权衡，决定质粒的命运，有利于中间成本质粒，而不是低成本和高成本的对应物。
Conjugative plasmids drive genetic diversity and evolution in microbial populations. Despite their prevalence, plasmids can impose long-term fitness costs on their hosts, altering population structure, growth dynamics, and evolutionary outcomes. In addition to long-term fitness costs, acquiring a new plasmid introduces an immediate, short-term perturbation to the cell. However, due to the transient nature of this plasmid acquisition cost, a quantitative understanding of its physiological manifestations, overall magnitudes, and population-level implications, remains unclear. To address this, here we track growth of single colonies immediately following plasmid acquisition. We find that plasmid acquisition costs are primarily driven by changes in lag time, rather than growth rate, for nearly 60 conditions covering diverse plasmids, selection environments, and clinical strains/species. Surprisingly, for a costly plasmid, clones exhibiting longer lag times also achieve faster recovery growth rates, suggesting an evolutionary tradeoff. Modeling and experiments demonstrate that this tradeoff leads to counterintuitive ecological dynamics, whereby intermediate-cost plasmids outcompete both their low and high-cost counterparts. These results suggest that, unlike fitness costs, plasmid acquisition dynamics are not uniformly driven by minimizing growth disadvantages. Moreover, a lag/growth tradeoff has clear implications in predicting the ecological outcomes and intervention strategies of bacteria undergoing conjugation. Plasmid acquisition imposes a transient burden on bacterial hosts. Here, authors show this burden results in a tradeoff between growth and lag that dictates plasmid fate, favoring intermediate cost plasmids over both low and high cost counterparts.
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影响因子：
4.5
作者：
Baharoglu Z;Bikard D;Mazel D
通讯作者：
Mazel D
DOI：
10.1073/pnas.1600974113
发表时间：
2016-07-19
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
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作者：
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影响因子：
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作者：
Lopatkin, Allison J.;Huang, Shuqiang;Smith, Robert P.;Srimani, Jaydeep K.;Sysoeva, Tatyana A.;Bewick, Sharon;Karig, David K.;You, Lingchong
通讯作者：
You, Lingchong
影响因子：
11.8
作者：
Maiden, MCJ
通讯作者：
Maiden, MCJ