Conjugative DNA transfer induces the bacterial SOS response and promotes antibiotic resistance development through integron activation.
Conjugative DNA transfer induces the bacterial SOS response and promotes antibiotic resistance development through integron activation.
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DOI：
10.1371/journal.pgen.1001165
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发表时间：
2010-10-21
期刊：
影响因子：
4.5
通讯作者：
Mazel D
中科院分区：
文献类型：
--
作者：
Baharoglu Z;Bikard D;Mazel D
接合是细菌种内和种间水平基因转移的一种机制。结合元件在许多细菌物种中起着重要作用，通过允许它们进化和适应这些不利的条件来面对抗生素的威胁。将接合质粒作为单链DNA转移至无质粒受体细胞。我们使用lacZ和gfp融合来解决缀合是否诱导SOS应答和整合子整合酶。SOS反应控制一系列负责DNA损伤修复的基因，这可能导致重组和诱变。在这份手稿中，我们表明，接合转移的ssDNA诱导细菌SOS应激反应，除非存在抗SOS因子，以减轻这种反应。我们还表明，整合子整合酶在此过程中上调，导致增加盒重排。此外，我们使用宽和窄宿主范围质粒获得的数据强烈表明，质粒转移，甚至流产，可以触发受体细胞中的染色体基因重排和转录开关。我们的研究结果强调了环境的重要性，集中不同的细菌群落作为反应器广泛的遗传适应的细菌。细菌在自然环境中交换DNA。接合过程是通过细胞接触将DNA从一个细菌转移到另一个细菌。共轭环状质粒被认为是适应性基因的穿梭载体和储存库。现在已经确定，这种横向基因转移起着至关重要的作用，特别是对于细菌之间的抗生素耐药性发展和传播。此外，整合子，移动的基因盒的平台，通过它们与接合抗性质粒的成功关联，在这一现象中起了重要作用。我们在这项研究中表明，接合转移质粒触发细菌应激反应-SOS反应-在受体细胞中，可以影响盒内容的整合子。已知SOS反应诱导各种基因组修饰。人类和动物病原体与环境细菌共存，在有利于DNA交换的小生境中。因此，接合期间的SOS诱导最有可能影响广泛的基因组。细菌SOS反应可能是一个合适的目标，共同治疗感染，以防止抗生素耐药性/适应基因的交换。
Conjugation is one mechanism for intra- and inter-species horizontal gene transfer among bacteria. Conjugative elements have been instrumental in many bacterial species to face the threat of antibiotics, by allowing them to evolve and adapt to these hostile conditions. Conjugative plasmids are transferred to plasmidless recipient cells as single-stranded DNA. We used lacZ and gfp fusions to address whether conjugation induces the SOS response and the integron integrase. The SOS response controls a series of genes responsible for DNA damage repair, which can lead to recombination and mutagenesis. In this manuscript, we show that conjugative transfer of ssDNA induces the bacterial SOS stress response, unless an anti-SOS factor is present to alleviate this response. We also show that integron integrases are up-regulated during this process, resulting in increased cassette rearrangements. Moreover, the data we obtained using broad and narrow host range plasmids strongly suggests that plasmid transfer, even abortive, can trigger chromosomal gene rearrangements and transcriptional switches in the recipient cell. Our results highlight the importance of environments concentrating disparate bacterial communities as reactors for extensive genetic adaptation of bacteria. Bacteria exchange DNA in their natural environments. The process called conjugation consists of DNA transfer by cell contact from one bacterium to another. Conjugative circular plasmids have been identified as shuttles and reservoirs for adaptive genes. It is now established that such lateral gene transfer plays an essential role, especially for the antibiotic resistance development and dissemination among bacteria. Moreover, integrons, platforms of mobile gene cassettes, have been instrumental in this phenomenon, through their successful association with conjugative resistance plasmids. We demonstrate in this study that the conjugative transfer of plasmids triggers a bacterial stress response—the SOS response—in recipient cells and can impact the cassette content of integrons. The SOS response is already known to induce various genome modifications. Human and animal pathogens cohabit with environmental bacteria, in niches which will favor DNA exchange. SOS induction during conjugation is thus most probably able to impact a wide range of genomes. Bacterial SOS response could then be a suitable target for co-treatment of infections in order to prevent exchange of antibiotic resistance/adaptation genes.
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影响因子：
64.8
作者：
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