The eIF3 interactome reveals the translasome, a supercomplex linking protein synthesis and degradation machineries.
The eIF3 interactome reveals the translasome, a supercomplex linking protein synthesis and degradation machineries.
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DOI：
10.1016/j.molcel.2009.09.026
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发表时间：
2009-10-09
期刊：
影响因子：
16
通讯作者：
Wolf, Dieter A.
中科院分区：
文献类型：
--
作者：
Sha, Zhe;Brill, Laurence M.;Cabrera, Rodrigo;Kleifeld, Oded;Scheliga, Judith S.;Glickman, Michael H.;Chang, Eric C.;Wolf, Dieter A.
eIF 3促进翻译起始，但对其在细胞中的全部活性知之甚少。在这里，我们采用亲和纯化和高灵敏度的LC-MS/MS来破译裂殖酵母eIF 3相互作用组，发现其含有230个蛋白质。eIF 3组装成一个大的超复合物，即蛋白酶体，它含有延伸因子、tRNA合成酶、40 S和60 S核糖体蛋白、伴侣蛋白和蛋白酶体。eIF 3还与核糖体生物合成因子和输入蛋白β Kap 123 p和Sal 3 p相关。我们的遗传数据表明，与两种importins-β的结合对于细胞生长是必不可少的，并且光漂白实验揭示了Sal 3 p在细胞内蛋白酶体组分之一蛋白酶体的核输入中的关键作用。我们的数据揭示了eIF 3相互作用组的宽度，并表明参与翻译起始，核糖体生物合成，翻译延伸，质量控制和运输的因素是物理连接，以促进有效的蛋白质合成。
eIF3 promotes translation initiation, but relatively little is known about its full range of activities in the cell. Here, we employed affinity purification and highly sensitive LC-MS/MS to decipher the fission yeast eIF3 interactome, which was found to contain 230 proteins. eIF3 assembles into a large supercomplex, the translasome, which contains elongation factors, tRNA-synthetases, 40S and 60S ribosomal proteins, chaperones, and the proteasome. eIF3 also associates with ribosome biogenesis factors and the importins-β Kap123p and Sal3p. Our genetic data indicated that the binding to both importins-β is essential for cell growth, and photobleaching experiments revealed a critical role for Sal3p in the nuclear import of one of the translasome constituents, the proteasome. Our data reveal the breadth of the eIF3 interactome and suggest that factors involved in translation initiation, ribosome biogenesis, translation elongation, quality control, and transport are physically linked to facilitate efficient protein synthesis.
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