Effects of lead and mercury on the blood proteome of children.
Effects of lead and mercury on the blood proteome of children.
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DOI：
10.1021/pr100204g
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发表时间：
2010-09-03
影响因子：
4.4
通讯作者：
Bendinskas, Kestutis G.
中科院分区：
文献类型：
--
作者：
Birdsall, Robert E.;Kiley, Michael P.;Segu, Zaneer M.;Palmer, Christopher D.;Madera, Milan;Gump, Brooks B.;MacKenzie, James A.;Parsons, Patrick J.;Mechref, Yehia;Novotny, Milos V.;Bendinskas, Kestutis G.
儿童接触重金属与各种生理和神经问题有关。这项研究的目的是利用蛋白质组学来加强对导致铅和汞暴露在远低于疾控中心指导方针的浓度下的健康问题的生化相互作用的理解。使用基于抗体的亲和色谱柱去除34名儿童的血浆和血清样本中最丰富的蛋白质，并使用两种不同的方法进行分析，即LC-MS/MS和双向电泳联用MALDI-TOF/MS和串联质谱仪。从LC-MS/MS和双向电泳法推断，载脂蛋白E与铅浓度(平均为1微克/分升)呈负相关，并经Western印迹分析证实。这与之前的研究结果一致，即载脂蛋白E基因对铅暴露个体的神经行为影响有缓和作用。LC-MS/MS鉴定了另外15个蛋白质，这些蛋白质在环境铅和汞存在的情况下表现出不同的表达差异。
Heavy metal exposure in children has been associated with a variety of physiological and neurological problems. The goal of this study was to utilize proteomics to enhance the understanding of biochemical interactions responsible for the health problems related to lead and mercury exposure at concentrations well below CDC guidelines. Blood plasma and serum samples from 34 children were depleted of their most abundant proteins using antibody-based affinity columns and analyzed using two different methods, LC-MS/MS and 2-D electrophoresis coupled with MALDI-TOF/MS and tandem mass spectrometry. Apolipoprotein E demonstrated an inverse significant association with lead concentrations (average being one microgram/deciliter) as deduced from LC-MS/MS and 2-D electrophoresis and confirmed by Western blot analysis. This coincides with prior findings that Apolipoprotein E genotype moderates neurobehavioral effects in individuals exposed to lead. Fifteen other proteins were identified by LC-MS/MS as proteins of interest exhibiting expressional differences in the presence of environmental lead and mercury.
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