Sustained delivery of chABC improves functional recovery after a spine injury.
Sustained delivery of chABC improves functional recovery after a spine injury.
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DOI：
10.1186/s12868-022-00734-8
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发表时间：
2022-10-28
期刊：
影响因子：
2.4
通讯作者：
Ramezani, Fatemeh
中科院分区：
文献类型：
--
作者：
Sharifi, Atousa;Zandieh, Ali;Behroozi, Zahra;Hamblin, Michael R.;Mayahi, Sara;Yousefifard, Mahmoud;Ramezani, Fatemeh
软骨素酶ABC （Chondroitinase ABC, chABC）是一种能够促进鼠脊髓损伤（SCI）再生从而促进功能恢复的酶。活性酶的降解和从病变处扩散是使用水凝胶作为支架将chABC运送到病变部位的原因。在这项荟萃分析中，我们研究了在支架或水凝胶中嵌入chABC对脊髓损伤后功能恢复的影响。根据chABC与脊髓损伤（SCI）相关的关键词检索数据库。进行一次和二次筛选以缩小研究目标和纳入标准，最后将数据纳入meta分析。采用Basso， Beattie, Bresnahan （BBB）测试脊髓损伤后功能恢复评分的标准均差对报道的研究结果进行分析。根据脊髓损伤模型、严重程度、移植类型、随访时间进行亚组分析。还规定了物品的质量控制。结果显示，9项研究中，在支架内嵌入chABC可显著提高动物模型脊髓损伤后功能恢复效率（SMD = 1.95; 95% CI 0.71-3.2; p = 0.002）。脊髓损伤模型、严重程度、损伤部位、移植类型、随访时间均不影响整体结果，且支架效应在所有病例中均不可忽视。然而，由于研究数量较少，这一结果并不具有结论性，需要进行更多的研究。本研究结果为chABC嵌入支架治疗脊髓损伤铺平了道路，并表明这种给药方式优于单独注射chABC。
Chondroitinase ABC (chABC) is an enzyme could improve regeneration and thereby improving functional recovery of spinal cord injury (SCI) in rodent models. Degradation of the active enzyme and diffusion away from the lesion are the causes of using hydrogels as a scaffold to deliver the chABC into the lesion site. In this meta-analysis, we investigated the effects of chABC embedded in a scaffold or hydrogel on the functional recovery after SCI. Databases were searched based on keywords related to chABC and spinal cord injury (SCI). Primary and secondary screening was performed to narrow down study objectives and inclusion criteria, and finally the data were included in the meta-analysis. The standard mean difference of the score of the functional recovery that measured by Basso, Beattie, Bresnahan (BBB) test after SCI was used to analyze the results of the reported studies. Subgroup analysis was performed based on SCI model, severity of SCI, transplantation type, and the follow-up time. Quality control of articles was also specified. The results showed that embedding chABC within the scaffold increased significantly the efficiency of functional recovery after SCI in animal models (SMD = 1.95; 95% CI 0.71–3.2; p = 0.002) in 9 studies. SCI model, severity of SCI, injury location, transplantation type, and the follow-up time did not affect the overall results and in all cases scaffold effect could not be ignored. However, due to the small number of studies, this result is not conclusive and more studies are needed. The results could pave the way for the use of chABC embedded in the scaffold for the treatment of SCI and show that this method of administration is superior to chABC injection alone.
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影响因子：
9.3
作者：
Dyck S;Kataria H;Alizadeh A;Santhosh KT;Lang B;Silver J;Karimi-Abdolrezaee S
通讯作者：
Karimi-Abdolrezaee S
影响因子：
4.2
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
2.2
作者：
Hassannejad, Z.;Sharif-Alhoseini, M.;Rahimi-Movaghar, V.
通讯作者：
Rahimi-Movaghar, V.
影响因子：
5.8
作者：
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通讯作者：
Omidian, Hamid