Presence of platelet-derived growth factor in normal and fibrotic lung is specifically associated with interstitial macrophages, while both interstitial macrophages and alveolar epithelial cells express the c-sis proto-oncogene.
Presence of platelet-derived growth factor in normal and fibrotic lung is specifically associated with interstitial macrophages, while both interstitial macrophages and alveolar epithelial cells express the c-sis proto-oncogene.
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正常和纤维化肺中血小板衍生生长因子的存在与间质巨噬细胞特异性相关，而间质巨噬细胞和肺泡上皮细胞均表达 c-sis 原癌基因。
DOI：
10.1165/ajrcmb/5.6.531
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发表时间：
1991
影响因子：
6.4
通讯作者：
Yves Martinet
中科院分区：
文献类型：
--
作者：
J. Vignaud;Mustapha Allam;N. Martinet;Michael Pech;François Plénat;Yves Martinet
正常的肺结构由间充质细胞及其细胞外基质产物的存在维持。这些细胞的缓慢正常周转在纤维化疾病中被破坏，导致间充质细胞及其细胞外基质的原位积累，导致进行性肺泡壁增厚。特发性肺纤维化（IPF）是一种慢性肺纤维化疾病，其特征是以巨噬细胞和多形核中性粒细胞为主的弥漫性间质和肺泡内炎症。通过支气管肺泡灌洗获得的肺泡巨噬细胞（AM）的评价先前表明，正常个体的AM自发释放少量血小板衍生生长因子（PDGF），一种间充质细胞的趋化和生长因子，而IPF患者的AM自发释放增加量的生物活性PDGF，表明其参与间充质细胞蓄积。然而，其他细胞如内皮细胞和血管平滑肌细胞也可以在体外释放PDGF。为了确定血小板源性生长因子在肺实质中的位置，使用抗血小板源性生长因子抗体通过免疫组织化学以及使用血小板源性生长因子A链和B链基因探针通过原位杂交对正常人和特发性肺纤维化患者的开放肺活检进行了检查。在正常肺和纤维化肺中，PDGF仅与间质巨噬细胞相关，而不与任何其他炎性细胞或间质细胞相关。此外，与正常肺相比，IPF中PDGF阳性巨噬细胞的百分比增加了3倍。此外，IPF肺的纤维化和非纤维化区域中PDGF阳性巨噬细胞的百分比相同。（250字处删节）
Normal lung structure is maintained by the presence of mesenchymal cells and their extracellular matrix products. The slow normal turnover of these cells is disrupted in fibrotic disorders, resulting in the in situ accumulation of mesenchymal cells and their extracellular matrix leading to a progressive alveolar wall thickening. Idiopathic pulmonary fibrosis (IPF) is a chronic fibrotic disorder of the lung characterized by a diffuse interstitial and intra-alveolar inflammation dominated by macrophages and polymorphonuclear neutrophils. Evaluation of alveolar macrophages (AM) obtained by bronchoalveolar lavage has previously shown that AM from normal individuals spontaneously release small amounts of platelet-derived growth factor (PDGF), a chemotactic and growth factor for mesenchymal cells, whereas AM from IPF patients spontaneously release increased amounts of biologically active PDGF, suggesting its involvement in mesenchymal cell accumulation. However, other cells such as endothelial cells and vascular smooth muscle cells can also release PDGF in vitro. In order to specify PDGF location in lung parenchyma, open lung biopsies from normal individuals and IPF patients were examined by immunohistochemistry using an anti-PDGF antibody and by in situ hybridization using PDGF A-chain and B-chain gene probes. In normal as well as in fibrotic lung, PDGF was only present in relation with interstitial macrophages but not with any other inflammatory cells or mesenchymal cells. Furthermore, the percentage of PDGF-positive macrophages in IPF was 3-fold increased in comparison to normal lung. In addition, the percentage of PDGF-positive macrophages was the same in fibrotic and nonfibrotic areas of IPF lungs.(ABSTRACT TRUNCATED AT 250 WORDS)
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多肽生长因子：在正常和异常细胞生长中的作用。
DOI：
10.1146/annurev.cb.03.110187.002303
发表时间：
1987
期刊：
Annual review of cell biology
影响因子：
--
作者：
Deuel,TF
通讯作者：
Deuel,TF
人恶性上皮细胞系血小板源性生长因子 (PDGF) 相关转录物的表达和生物活性 PDGF 样蛋白的合成。
DOI：
10.1172/jci113712
发表时间：
1988
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Sariban,E;Sitaras,NM;Antoniades,HN;Kufe,DW;Pantazis,P
通讯作者：
Pantazis,P
血小板衍生生长因子：正常细胞和转化细胞中的结构、功能和作用。
DOI：
10.1016/b978-0-12-152826-3.50012-7
发表时间：
1985
期刊：
Current topics in cellular regulation
影响因子：
--
作者：
Deuel,TF;Tong,BD;Huang,JS
通讯作者：
Huang,JS
影响因子：
2.7
作者：
COX, KH;DELEON, DV;ANGERER, RC
通讯作者：
ANGERER, RC
DOI：
10.1073/pnas.84.17.6020
发表时间：
1987-09-01
影响因子：
11.1
作者：
ASSOIAN, RK;FLEURDELYS, BE;SPORN, MB
通讯作者：
SPORN, MB