KRE genes are required for beta-1,6-glucan synthesis, maintenance of capsule architecture and cell wall protein anchoring in Cryptococcus neoformans.
KRE genes are required for beta-1,6-glucan synthesis, maintenance of capsule architecture and cell wall protein anchoring in Cryptococcus neoformans.
复制标题
DOI：
10.1111/j.1365-2958.2010.07119.x
复制
发表时间：
2010-04
影响因子：
3.6
通讯作者：
Lodge JK
中科院分区：
文献类型：
--
作者：
Gilbert NM;Donlin MJ;Gerik KJ;Specht CA;Djordjevic JT;Wilson CF;Sorrell TC;Lodge JK
多糖β-1,6-葡聚糖是新型隐球菌细胞壁的主要成分，但其在这种真菌病原体中的作用尚未被研究。我们鉴定并鉴定了7个属于KRE家族的基因，这些基因被认为参与了β-1,6-葡聚糖的合成。H99缺失突变体kre5Δ和kre6Δskn1Δ含有较少的细胞壁β-1,6-葡聚糖，生长缓慢，形态异常，对环境和化学应激高度敏感，在小鼠吸入感染模型中无毒。这两个突变体显示出细胞壁壳聚糖和外多糖胶囊的改变，这是隐球菌主要的毒力决定因素。隐球菌细胞壁完整性和毒力所需的gpi锚定磷脂酶B1 （Plb1）酶的细胞壁含量在kre5Δ和kre6Δskn1Δ中降低。我们的研究结果表明，KRE5、KRE6和SKN1参与了新生隐球菌细胞壁中β-1,6-葡聚糖的合成、细胞壁完整性的维持以及甘露蛋白和已知隐球菌毒力因子的保留。这项研究为进一步研究这种丰富的细胞壁聚合物的功能奠定了基础。
The polysaccharide β-1,6-glucan is a major component of the cell wall of Cryptococcus neoformans, but its function has not been investigated in this fungal pathogen. We have identified and characterized seven genes, belonging to the KRE family, which are putatively involved in β-1,6-glucan synthesis. The H99 deletion mutants kre5Δ and kre6Δskn1Δ contained less cell wall β-1,6-glucan, grew slowly with an aberrant morphology, were highly sensitive to environmental and chemical stress and were avirulent in a mouse inhalation model of infection. These two mutants displayed alterations in cell wall chitosan and the exopolysaccharide capsule, a primary cryptococcal virulence determinant. The cell wall content of the GPI-anchored phospholipase B1 (Plb1) enzyme, which is required for cryptococcal cell wall integrity and virulence, was reduced in kre5Δ and kre6Δskn1Δ. Our results indicate that KRE5, KRE6 and SKN1 are involved in β-1,6-glucan synthesis, maintenance of cell wall integrity and retention of mannoproteins and known cryptococcal virulence factors in the cell wall of C. neoformans. This study sets the stage for future investigations into the function of this abundant cell wall polymer.
登录
查看更多内容
影响因子：
6.4
作者：
Chen, SCA;Muller, M;Sorrell, TC
通讯作者：
Sorrell, TC
影响因子：
4.8
作者：
Aimanianda, Vishukumar;Clavaud, Cecile;Latge, Jean-Paul
通讯作者：
Latge, Jean-Paul
影响因子：
3
作者：
Goins, Chelsey L.;Gerik, Kimberly J.;Lodge, Jennifer K.
通讯作者：
Lodge, Jennifer K.
影响因子：
2.8
作者：
Hoch, HC;Galvani, CD;Turner, JN
通讯作者：
Turner, JN
影响因子：
4.1
作者：
Djordjevic, JT;Del Poeta, M;Wright, LC
通讯作者：
Wright, LC