An analysis of diet quality, how it controls fatty acid profiles, isotope signatures and stoichiometry in the malaria mosquito Anopheles arabiensis.
An analysis of diet quality, how it controls fatty acid profiles, isotope signatures and stoichiometry in the malaria mosquito Anopheles arabiensis.
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DOI：
10.1371/journal.pone.0045222
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Gilles J
中科院分区：
文献类型：
--
作者：
Hood-Nowotny R;Schwarzinger B;Schwarzinger C;Soliban S;Madakacherry O;Aigner M;Watzka M;Gilles J
了解蚊子生态学的基本机制将确保有效的病媒管理，并有助于实现疟疾控制的总体目标。苔藓植物种群对环境变化的响应表现出高度的种群可塑性。然而，控制种群规模和繁殖力的主要因素在很大程度上是未知的。幼虫的栖息地和饮食在随后的蚊子健康中起着至关重要的作用。开发最有竞争力的昆虫不育昆虫技术方案需要一个“生产”为导向的角度来看，推导出最有效的幼虫饮食配方，从这个过程中获得的信息为我们提供了一些洞察机制和过程发生在自然原生蚊子栖息地。脂肪酸概况和从头或直接同化途径，整个个别蚊子饲养的范围内幼虫的饮食，使用热解气相色谱/质谱法测定。我们使用元素分析和同位素比值质谱法来测量个体全身碳，氮和磷的值，并评估饮食质量对随后的人口化学计量，大小，质量和同位素特征的影响。饮食有最大的影响，脂肪酸（FA）配置文件的蚊子，表现出高度的饮食路线，特征的通才馈线。观察到一些重要的FA的从头合成。在长期阶段，土壤中的碳氮化学计量比是固定的。食物中的氮含量对蚊子的大小有显著的影响，而磷则是一个灵活的库，限制了整个种群的大小。直接路由的脂肪酸是显而易见的，但有无处不在的从头合成，这表明蚊子幼虫是有能力的通才饲养者，能够生存的饮食与不同的特点。得出的结论是，幼虫饮食中的氮可用性控制teneral蚊子的大小和teneral CN比是一个性别和物种特异性的固定参数。这一发现对整体蚊子竞争力和环境管理具有重要意义。
Knowing the underlying mechanisms of mosquito ecology will ensure effective vector management and contribute to the overall goal of malaria control. Mosquito populations show a high degree of population plasticity in response to environmental variability. However, the principle factors controlling population size and fecundity are for the most part unknown. Larval habitat and diet play a crucial role in subsequent mosquito fitness. Developing the most competitive insects for sterile insect technique programmes requires a “production” orientated perspective, to deduce the most effective larval diet formulation; the information gained from this process offers us some insight into the mechanisms and processes taking place in natural native mosquito habitats. Fatty acid profiles and de-novo or direct assimilation pathways, of whole-individual mosquitoes reared on a range of larval diets were determined using pyrolysis gas chromatograph/mass spectrometry. We used elemental analysis and isotope ratio mass spectrometry to measure individual-whole-body carbon, nitrogen and phosphorous values and to assess the impact of dietary quality on subsequent population stoichiometry, size, quality and isotopic signature. Diet had the greatest impact on fatty acid (FA) profiles of the mosquitoes, which exhibited a high degree of dietary routing, characteristic of generalist feeders. De-novo synthesis of a number of important FAs was observed. Mosquito C:N stoichiometry was fixed in the teneral stage. Dietary N content had significant influence on mosquito size, and P was shown to be a flexible pool which limited overall population size. Direct routing of FAs was evident but there was ubiquitous de-novo synthesis suggesting mosquito larvae are competent generalist feeders capable of survival on diet with varying characteristics. It was concluded that nitrogen availability in the larval diet controlled teneral mosquito size and that teneral CN ratio is a sex- and species-specific fixed parameter. This finding has significant implications for overall mosquito competitiveness and environmental management.
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影响因子：
2.2
作者：
Benedict, Mark Q.;Hood-Nowotny, Rebecca C.;Wilkins, Elien E.
通讯作者：
Wilkins, Elien E.
影响因子：
2.1
作者：
Gilles, J. R. L.;Lees, R. S.;Benedict, M. Q.
通讯作者：
Benedict, M. Q.
影响因子：
4.1
作者：
GOLBERG, L;DEMEILLON, B
通讯作者：
DEMEILLON, B
影响因子：
5
作者：
DENIRO, MJ;EPSTEIN, S
通讯作者：
EPSTEIN, S
影响因子：
4.2
作者：
DADD, RH
通讯作者：
DADD, RH