A distinctive layering pattern of mouse dentate granule cells is generated by developmental and adult neurogenesis.
A distinctive layering pattern of mouse dentate granule cells is generated by developmental and adult neurogenesis.
复制标题
DOI：
10.1002/cne.22489
复制
发表时间：
2010-11-15
影响因子：
2.5
通讯作者：
Gage, Fred H.
中科院分区：
文献类型：
--
作者：
Mathews, Emily A.;Morgenstern, Nicolas A.;Piatti, Veronica C.;Zhao, Chunmei;Jessberger, Sebastian;Schinder, Alejandro F.;Gage, Fred H.
关键词：
在哺乳动物的海马齿状回中，新的神经元在整个生命过程中不断增加。在胚胎和出生后早期的发育过程中，齿状回形成一种由外向内的分层模式，这种模式可能会一直延伸到成年。在这项工作中，我们旨在系统地量化不同年龄产生的齿状颗粒细胞的相对位置。我们使用5‘-溴-2’-脱氧尿苷(BrdU)和逆转录病毒方法对出生于胚胎、出生后早期和成年海马区的细胞进行检测，并评估它们在成年小鼠颗粒细胞层中的最终位置。我们还量化了发育中和成年出生的神经前体细胞队列，这些神经前体细胞对成体前体细胞库做出了贡献。我们的数据证实，齿状回由外向内的分层一直持续到成年期，早期出生的细胞构成了成年齿状回的大部分。我们还发现，成年齿状回中相当一部分分裂细胞来自早期分裂细胞，并保留了BrdU，这表明海马祖细胞亚群从早期发育开始就很少分裂。
New neurons are continuously added throughout life to the dentate gyrus of the mammalian hippocampus. During embryonic and early postnatal development, the dentate gyrus is formed in an outside-in layering pattern that may extend through adulthood. In this work we aimed to systematically quantify the relative position of dentate granule cells generated at different ages. We used 5’-bromo-2’-deoxyuridine (BrdU) and retroviral methodologies to birth-date cells born in the embryonic, early postnatal and adult hippocampus and assessed their final position in the adult mouse granule cell layer. We also quantified both developmental and adult-born cohorts of neural progenitor cells that contribute to the pool of adult progenitor cells. Our data confirm that the outside-in layering of the dentate gyrus continues through adulthood and that early-born cells constitute most of the adult dentate gyrus. We also found that a substantial fraction of the dividing cells in the adult dentate gyrus were derived from early-dividing cells and retained BrdU, suggesting that a subpopulation of hippocampal progenitors divides infrequently from early development on.
登录
查看更多内容
影响因子：
82.9
作者：
Eriksson, PS;Perfilieva, E;Gage, FH
通讯作者：
Gage, FH
影响因子：
2.5
作者：
Dayer, AG;Ford, AA;Cameron, HA
通讯作者：
Cameron, HA
影响因子：
2.5
作者：
BAYER, SA;ALTMAN, J
通讯作者：
ALTMAN, J
DOI：
10.1016/j.brainresprot.2004.09.003
发表时间：
2004-11-01
期刊：
BRAIN RESEARCH PROTOCOLS
影响因子：
--
作者：
Bonthius, DJ;McKim, R;Pantazis, NJ
通讯作者：
Pantazis, NJ
影响因子：
5.3
作者：
Ciaroni, S;Cecchini, T;Del Grande, P
通讯作者：
Del Grande, P