Expression of GLUT1 in stratified squamous epithelia and oral carcinoma from humans and rats
Expression of GLUT1 in stratified squamous epithelia and oral carcinoma from humans and rats
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GLUT1在人和大鼠复层鳞状上皮和口腔癌中的表达
DOI：
10.1111/j.1699-0463.1997.tb05051.x
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发表时间：
1997
期刊：
影响因子：
2.8
通讯作者：
E. Dabelsteen
中科院分区：
文献类型：
--
作者：
M. Voldstedlund;E. Dabelsteen
大多数细胞表达促进性葡萄糖转运蛋白。四种异构体（GLUT1‐4）通过质膜运输D -葡萄糖，显示出特定的组织分布，这是葡萄糖代谢中组织特异性模式的基础。GLUT1在组织屏障（如血脑屏障）中高水平表达，该异构体已被认为是这种屏障的一个指标。在没有这种组织屏障存在的人表皮的基底层中发现了GLUT1。为了进一步阐明这些问题，我们用间接免疫荧光显微镜检查了GLUT1和GLUT4在皮肤、不同类型的大鼠和人口腔黏膜以及人口腔癌中的分布。结果表明，GLUT1在不同层状鳞状上皮的基底层和旁基层表达，在角化亚型和非角化亚型之间存在一定差异。GLUT1也在小唾液腺的导管上皮细胞和肌上皮细胞以及原膜的神经周围鞘中表达，此外在口腔癌细胞中也表达。GLUT4在所有组织中均未表达。这种GLUT1的分布不符合将GLUT1作为组织屏障的一般指标的观点。相反，我们的研究结果支持了普遍存在的，但对鳞状分层上皮中葡萄糖代谢的有限认识，这种代谢被认为主要依赖于糖酵解，特别是在基底层。高水平表达似乎仅限于没有糖原储存的角化细胞。
Most cells express facilitative glucose transporters. Four isoforms (GLUT1‐4) transporting D‐glucose across the plasma membrane show a specific tissue distribution, which is the basis for tissue‐specific patterns in glucose metabolism. GLUT1 is expressed at high levels in tissue barriers such as the blood‐brain barrier, and this isoform has been suggested as an indicator of such barriers. GLUT1 has been found in basal layers of human epidermis where no such tissue barrier is present. To further clarify these issues, we examined the distribution of GLUT1 and GLUT4 in skin, different types of oral mucosa from rat and man, and a human oral careinoma by indirect immunofluorescence microscopy. The results showed that GLUT1 was expressed in the basal and parabasal layers of the different stratified squamous epithelia, with some variations between keratinized and non‐ keratinized subtypes. GLUT1 was also expressed in ductal‐ and myoepithelial cells of minor salivary glands and perineural sheath located in the lamina propra, and furthermore in the cells of an oral careinoma. GLUT4 was not expressed in any of the tissues examined. This distribution of GLUT1 does not fit with the idea of GLUT1 as a general indicator of tissue barriers. In contrast, our results support the prevailing, but limited knowledge of glucose metabolism in squamous stratified epithelia, a metabolism believed to depend mostly on glycolysis, especially in the basal layers. High‐level expression seemed to be confined to keratinocytes without glycogen stores.
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DOI：
--
发表时间：
1995
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
C. Heilig;C. Zaloga;M. Lee;X. Zhao;B. Riser;F. Brosius;P. Cortés
通讯作者：
C. Heilig;C. Zaloga;M. Lee;X. Zhao;B. Riser;F. Brosius;P. Cortés
口腔上皮屏障功能的细胞和分子基础。
影响因子：
2.7
作者：
Wertz,PW;Squier,CA
通讯作者：
Squier,CA
葡萄糖转运蛋白在血眼屏障处具有“闭塞”连接的细胞中含量丰富。
DOI：
10.1073/pnas.87.11.4261
发表时间：
1990
影响因子：
11.1
作者：
Harik,SI;Kalaria,RN;Whitney,PM;Andersson,L;Lundahl,P;Ledbetter,SR;Perry,G
通讯作者：
Perry,G
在蛋白激酶 C 缺失的情况下，转化可刺激葡萄糖转运蛋白基因表达。
DOI：
10.1073/pnas.86.21.8252
发表时间：
1989
影响因子：
11.1
作者：
Hiraki,Y;GarciadeHerreros,A;Birnbaum,MJ
通讯作者：
Birnbaum,MJ
DOI：
10.1016/s0021-9258(19)36489-0
发表时间：
1993-09
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
G. Bell;C. Burant;J. Takeda;G. Gould
通讯作者：
G. Bell;C. Burant;J. Takeda;G. Gould