Macrophage colony-stimulating factor expressed in non-cancer tissues provides predictive powers for recurrence in hepatocellular carcinoma.
Macrophage colony-stimulating factor expressed in non-cancer tissues provides predictive powers for recurrence in hepatocellular carcinoma.
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DOI：
10.3748/wjg.v22.i39.8779
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发表时间：
2016-10-21
影响因子：
4.3
通讯作者：
Sun C
中科院分区：
文献类型：
--
作者：
Kono H;Fujii H;Furuya S;Hara M;Hirayama K;Akazawa Y;Nakata Y;Tsuchiya M;Hosomura N;Sun C
目的探讨巨噬细胞集落刺激因子（M-CSF）在肝细胞癌（HCC）术后的作用。评估了肝脏（包括肿瘤和瘤周肝组织）中M-CSF的表达、M2巨噬细胞（MΦ）的分布和血管生成。这些因素的预后能力进行了评估。用含有M-CSF的培养基培养小鼠分离的肝MΦ或单核细胞。评估培养基中血管内皮生长因子（VEGF）的浓度。此外，研究了M-CSF成熟的肝MΦ对血管内皮细胞（VEC）增殖的作用。M-CSF和CD 163在瘤周区域的表达之间存在强相关性。此外，具有高密度M-CSF、CD 163或CD 31的组显示出比低密度组显著更短的复发时间（TTR）。多因素分析显示，M-CSF或肝M2 M Φ在瘤周区域的表达是导致TTR缩短的最关键因素。M-CSF和M2 M Φs在瘤周的表达对总生存期有较好的预测能力。与单核细胞相比，培养液中的VEGF值在肝MΦ中显著更高。当培养基中存在M-CSF时，VEC在与肝MΦ共培养的细胞中增殖最大。M-CSF增加肝癌发生，最可能是通过增强来自肝脏MΦ的血管生成因子，并且可能是治疗HCC的有用靶点。
To investigate the role of macrophage colony-stimulating factor (M-CSF) in patients with hepatocellular carcinoma (HCC) after surgery. Expression of M-CSF, distribution of M2 macrophages (MΦs), and angiogenesis were assessed in the liver, including tumors and peritumoral liver tissues. The prognostic power of these factors was assessed. Mouse isolated hepatic MΦs or monocytes were cultured with media containing M-CSF. The concentration of vascular endothelial growth factor (VEGF) in media was assessed. Furthermore, the role of the M-CSF-matured hepatic MΦs on proliferation of the vascular endothelial cell (VEC) was investigated. A strong correlation between the expressions of M-CSF and CD163 was observed in the peritumoral area. Also, groups with high density of M-CSF, CD163 or CD31 showed a significantly shorter time to recurrence (TTR) than low density groups. Multivariate analysis revealed the expression of M-CSF or hepatic M2MΦs in the peritumoral area as the most crucial factor responsible for shorter TTR. Moreover, the expression of M-CSF and hepatic M2MΦs in the peritumoral area had better predictable power of overall survival. Values of VEGF in culture media were significantly greater in the hepatic MΦs compared with the monocytes. Proliferation of the VEC was greatest in the cells co-cultured with hepatic MΦs when M-CSF was present in media. M-CSF increases hepatocarcinogenesis, most likely by enhancing an angiogenic factor derived from hepatic MΦ and could be a useful target for therapy against HCC.
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影响因子：
4.4
作者：
KACINSKI, BM
通讯作者：
KACINSKI, BM
DOI：
10.1152/ajpgi.2000.278.3.g467
发表时间：
2000-03-01
影响因子：
4.5
作者：
Kono, H;Enomoto, N;Thurman, RG
通讯作者：
Thurman, RG
影响因子：
51.1
作者：
Bockhorn, Maximilian;Jain, Rakesh K.;Munn, Lance L.
通讯作者：
Munn, Lance L.
影响因子：
64.5
作者：
Fukumura, D;Xavier, R;Seed, B
通讯作者：
Seed, B
DOI：
10.1067/s0002-9378(03)00674-4
发表时间：
2003-11-01
影响因子：
9.8
作者：
Hayashi, M;Ohkura, T;Inaba, N
通讯作者：
Inaba, N