Novel targets identified by integrated proteomic and phosphoproteomic analysis in spermatogenesis of swamp buffalo (Bubalus bubalis).
Novel targets identified by integrated proteomic and phosphoproteomic analysis in spermatogenesis of swamp buffalo (Bubalus bubalis).
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通过综合蛋白质组学和磷酸蛋白质组学分析确定沼泽水牛（Bubalus bubalis）精子发生中的新靶点
DOI：
10.1038/s41598-020-72353-4
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发表时间：
2020-09-24
影响因子：
4.6
通讯作者：
Zhang M
中科院分区：
文献类型：
--
作者：
Huang YL;Zhang PF;Fu Q;He WT;Xiao K;Zhang M
为了了解精子发生的机制，采用串联质量标签（TMT）结合液相色谱-串联质谱（LC-MS/MS）分析了青春期前和青春期沼泽布法罗（Bubalus bubalis）睾丸的蛋白质组和磷酸化蛋白质组。与青春期睾丸相比，青春期前睾丸中有80个蛋白质过度表达，148个蛋白质表达不足，139个和142个蛋白质位点分别具有较高和较低的磷酸化水平，其中一些蛋白质与生殖过程相关，如有性生殖、精子发生、受精和精子细胞发育。特别是，外致密纤维蛋白1（ODF 1），蛋白质大漩涡同源物（MAEL），肌动蛋白样蛋白7 B（ACTL 7 B），酪氨酸-（Y）-磷酸化调节（CABYR），和三方基序包含36（TRIM 36）在蛋白质组和磷酸化蛋白质组水平随着年龄的上调。将蛋白质组和磷酸化蛋白质组相结合可以有效地研究布法罗睾丸的蛋白质/磷酸化模式。这些数据为布法罗睾丸精子发生的复杂网络提供了新的调控候选者和证据，也为探索哺乳动物精子发生的生理机制提供了重要资源。
To understand mechanisms of spermatogenesis, the proteome and the phosphoproteome in prepubertal and pubertal swamp buffalo (Bubalus bubalis) testes were analyzed using tandem mass tag (TMT) coupled with liquid chromatography-tandem mass spectrometry (LC–MS/MS). In prepubertal testes, 80 proteins were overexpressed, 148 proteins were underexpressed, and 139 and 142 protein sites had higher and lower phosphorylation, respectively, compared to the levels in pubertal testes. Several of these proteins were associated with reproductive processes such as sexual reproduction, spermatogenesis, fertilization, and spermatid development. In particular, outer dense fiber protein 1 (ODF1), protein maelstrom homolog (MAEL), actin-like protein 7B (ACTL7B), tyrosine-(Y)-phosphorylation regulated (CABYR), and tripartite motif containing 36 (TRIM36) were upregulated with age at both the proteome and phosphoproteome levels. Combining proteome and phosphoproteome analysis can be effectively applied to study the protein/phosphorylation patterns of buffalo testes. These data provide new regulatory candidates and evidence for a complex network in spermatogenesis in buffalo testes, and serve as an important resource for exploring the physiological mechanism of spermatogenesis in mammals.
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影响因子：
64.5
作者：
Huttlin EL;Jedrychowski MP;Elias JE;Goswami T;Rad R;Beausoleil SA;Villén J;Haas W;Sowa ME;Gygi SP
通讯作者：
Gygi SP
影响因子：
13.6
作者：
Li MW;Mruk DD;Cheng CY
通讯作者：
Cheng CY
DOI：
10.1073/pnas.0406325101
发表时间：
2004-11-23
影响因子：
11.1
作者：
Khalil, AM;Boyar, FZ;Driscoll, DJ
通讯作者：
Driscoll, DJ
影响因子：
64.5
作者：
KEMPHUES, KJ;RAFF, EC;KAUFMAN, TC
通讯作者：
KAUFMAN, TC
影响因子：
64.5
作者：
KEMPHUES, KJ;KAUFMAN, TC;RAFF, EC
通讯作者：
RAFF, EC