Comparison of MALDI-TOF MS, housekeeping gene sequencing, and 16S rRNA gene sequencing for identification of Aeromonas clinical isolates.
Comparison of MALDI-TOF MS, housekeeping gene sequencing, and 16S rRNA gene sequencing for identification of Aeromonas clinical isolates.
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DOI：
10.3349/ymj.2015.56.2.550
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发表时间：
2015-03
影响因子：
2.4
通讯作者：
Lee K
中科院分区：
文献类型：
--
作者：
Shin HB;Yoon J;Lee Y;Kim MS;Lee K
气单胞菌属是一种众所周知会引起严重临床疾病的病原体，范围从胃肠炎到败血症。准确鉴定A. Alzheimer，A. cacetyl和A. veronii对于患者的护理非常重要。然而，使用传统方法进行物种识别仍然很困难。本研究的目的是比较不同的方法，确定气单胞菌在物种水平的准确性：生化方法，基质辅助激光解吸电离质谱飞行时间（MALDI-TOF MS），16 S rRNA测序，管家基因测序（gyrB，rpoB）。我们分析了1996年至2012年期间从韩国一所大学医院的患者中回收的65株气单胞菌分离株。从冷冻状态下回收的分离株，并使用以下四种方法进行测试：常规的生化方法，16 S rRNA测序，管家基因测序与系统发育分析，和MALDI-TOF MS。常规的生化方法和16 S rRNA测序鉴定气单胞菌属水平非常准确，虽然物种水平的鉴定是不令人满意的。MALDI-TOF MS系统在种水平上正确鉴定了60株（92.3%），在属水平上正确鉴定了另外4株（6.2%）。总体而言，管家基因测序与系统发育分析被认为是最准确的识别气单胞菌在物种水平。最准确的鉴定气单胞菌属的方法是通过管家基因测序，尽管高成本和技术难度阻碍了其在临床环境中的使用。临床实验室需要一种易于使用的鉴定方法，MALDI-TOF MS可能是一个强有力的候选者。
The genus Aeromonas is a pathogen that is well known to cause severe clinical illnesses, ranging from gastroenteritis to sepsis. Accurate identification of A. hydrophila, A. caviae, and A. veronii is important for the care of patients. However, species identification remains difficult using conventional methods. The aim of this study was to compare the accuracy of different methods of identifying Aeromonas at the species level: a biochemical method, matrix-assisted laser desorption ionization mass spectrometry-time of flight (MALDI-TOF MS), 16S rRNA sequencing, and housekeeping gene sequencing (gyrB, rpoB). We analyzed 65 Aeromonas isolates recovered from patients at a university hospital in Korea between 1996 and 2012. The isolates were recovered from frozen states and tested using the following four methods: a conventional biochemical method, 16S rRNA sequencing, housekeeping gene sequencing with phylogenetic analysis, and MALDI-TOF MS. The conventional biochemical method and 16S rRNA sequencing identified Aeromonas at the genus level very accurately, although species level identification was unsatisfactory. MALDI-TOF MS system correctly identified 60 (92.3%) isolates at the species level and an additional four (6.2%) at the genus level. Overall, housekeeping gene sequencing with phylogenetic analysis was found to be the most accurate in identifying Aeromonas at the species level. The most accurate method of identification of Aeromonas to species level is by housekeeping gene sequencing, although high cost and technical difficulty hinder its usage in clinical settings. An easy-to-use identification method is needed for clinical laboratories, for which MALDI-TOF MS could be a strong candidate.
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影响因子：
3.7
作者：
Puthucheary SD;Puah SM;Chua KH
通讯作者：
Chua KH
影响因子：
9.4
作者：
Mellmann, A.;Cloud, J.;Harmsen, D.
通讯作者：
Harmsen, D.
DOI：
10.1099/ijs.0.03048-0
发表时间：
2004-09-01
影响因子：
2.8
作者：
Soler, L;Yáñez, MA;Martínez-Murcia, AJ
通讯作者：
Martínez-Murcia, AJ
DOI：
10.1099/ijs.0.02443-0
发表时间：
2003-05-01
影响因子：
2.8
作者：
Yáñez, MA;Catalán, V;Martínez-Murcia, AJ
通讯作者：
Martínez-Murcia, AJ
DOI：
10.1099/00207713-42-3-412
发表时间：
1992-07-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
MARTINEZMURCIA, AJ;BENLLOCH, S;COLLINS, MD
通讯作者：
COLLINS, MD