Mechanical characterization of compressible chromatographic particles
Mechanical characterization of compressible chromatographic particles
复制标题
可压缩色谱颗粒的机械表征
DOI：
10.1016/j.powtec.2017.07.043
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发表时间：
2017
影响因子：
5.2
通讯作者：
Hekmat D
中科院分区：
文献类型：
--
作者：
Dorn M;Schilde C;Burmeister CF;Hekmat D
在填充床色谱柱的操作过程中，多孔色谱颗粒的机械性质决定了整体宏观压缩行为。多孔颗粒是由固体基质和间隙液组成的两相材料。采用高分辨率显微操作技术，对琼脂糖和甲基丙烯酸酯两种聚合物色谱颗粒（平均粒径分别为60 μm和83 μm）的阵列进行了力学表征。研究了压缩速度和压缩量对压缩效果的影响。这两种颗粒类型表现出明显的时间依赖性的压缩行为和力松弛。长时间压缩载荷松弛实验揭示了两种不同的时间依赖性机制。结果表明，描述任一机制的特征力弛豫时间相差13-31倍。短时准静态压缩实验可以很好地用麦克斯韦型标准线性固体模型来描述，其中力松弛时间与较短的松弛时间相匹配。据推测，在短时间内发生的力松弛归因于多孔弹性材料行为，在较长时间内归因于粘弹性材料行为。这使得基本的颗粒特性，即瞬时和放松的颗粒剪切和杨氏模量，泊松比和恢复系数的测定。
During operation of packed-bed chromatography columns, the mechanical properties of the porous chromatographic particles determine the overall macroscopic compression behavior. The porous particles are biphasic materials consisting of a solid matrix and interstitial fluid. Mechanical characterization of selected arrays of two types of polymeric chromatographic particles made of agarose and methacrylate with a mean particle diameter of 60 μm and 83 μm, respectively, was carried out by applying a high-resolution micromanipulation technique. The effect of compression speed and displacement level was investigated. Both particle types showed pronounced time-dependent compression behavior and force relaxation. Long-time compression load relaxation experiments revealed that two different time-dependent mechanisms were involved. It was shown that the characteristic force relaxation times describing either mechanism differed by a factor of 13–31. Short-time quasistatic compression experiments were excellently described by a Maxwell-type standard linear solid model in which the force relaxation time matched the shorter relaxation time. It was assumed, that force relaxation occurring at short time is ascribed to poroelastic material behavior and at longer times to viscoelastic material behavior. This enabled the determination of fundamental particle properties, i.e. instantaneous and relaxed particle shear and Young's moduli, Poisson's ratio, and coefficient of restitution.
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影响因子：
4.7
作者：
Nguyen, V. B.;Wang, C. X.;Zhang, Z.
通讯作者：
Zhang, Z.
影响因子：
4.1
作者：
Müller, E;Chung, JT;Sprauer, A
通讯作者：
Sprauer, A
制备型填充色谱床瞬态流体动力学记忆行为的宏观研究。
DOI：
10.1016/j.chroma.2010.12.092
发表时间：
2011
期刊：
Journal of chromatography. A
影响因子：
--
作者：
D. Hekmat;R. Mornhinweg;G. Bloch;Yu Sun;P. Jeanty;M. Neubert;D. Weuster‐Botz
通讯作者：
D. Weuster‐Botz
使用过程数据评估生产规模蛋白质纯化柱的色谱性能
影响因子：
2.9
作者：
Tina M. Larson;Jeff S. Davis;H. Lam;J. Cacia
通讯作者：
J. Cacia
影响因子：
2.9
作者：
Keener, RN;Maneval, JE;Fernandez, EJ
通讯作者：
Fernandez, EJ