Global characterization of Artemisia annua glandular trichome transcriptome using 454 pyrosequencing.
Global characterization of Artemisia annua glandular trichome transcriptome using 454 pyrosequencing.
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DOI：
10.1186/1471-2164-10-465
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发表时间：
2009-10-09
期刊：
影响因子：
4.4
通讯作者：
Guo D
中科院分区：
文献类型：
--
作者：
Wang W;Wang Y;Zhang Q;Qi Y;Guo D
在许多植物物种中，腺毛产生各种各样的具有重要商业价值的次生代谢产物。最著名的抗疟疾药物青蒿素是一种倍半萜内酯，产于黄花蒿的腺毛中。然而，只有有限的基因组信息，目前可在这种非模式植物物种。我们提出了一个全球性的表征A。使用454焦磷酸测序的一年生腺毛转录组。使用来自腺毛的两个标准化cDNA集合的测序运行产生406，044个表达的序列标签（平均长度= 210个核苷酸），其组装成42，678个重叠群和147，699个单例。进行第二次测序运行仅使鉴定的基因数量增加了约30%，表明大规模平行焦磷酸测序提供了A.一年生毛状体转录组。通过对NCBI非冗余蛋白质数据库的BLAST搜索，将推定的功能分配给超过28，573个单基因，包括以前未描述的可能参与倍半萜生物合成的酶。与来自其他植物物种的毛状体收集的EST的比较显示，在不同的植物物种的共同功能类别的表达基因。RT-PCR分析证实了所选的unigenes和新的转录本在A.年轮腺毛。与萜类化合物和类黄酮生物合成酶对应的重叠群的存在表明A.一年生腺毛对腺毛中表达基因的全面研究将有助于发现新的基因，并有助于阐明青蒿素代谢和腺毛功能的调控机制。annua。
Glandular trichomes produce a wide variety of commercially important secondary metabolites in many plant species. The most prominent anti-malarial drug artemisinin, a sesquiterpene lactone, is produced in glandular trichomes of Artemisia annua. However, only limited genomic information is currently available in this non-model plant species. We present a global characterization of A. annua glandular trichome transcriptome using 454 pyrosequencing. Sequencing runs using two normalized cDNA collections from glandular trichomes yielded 406,044 expressed sequence tags (average length = 210 nucleotides), which assembled into 42,678 contigs and 147,699 singletons. Performing a second sequencing run only increased the number of genes identified by ~30%, indicating that massively parallel pyrosequencing provides deep coverage of the A. annua trichome transcriptome. By BLAST search against the NCBI non-redundant protein database, putative functions were assigned to over 28,573 unigenes, including previously undescribed enzymes likely involved in sesquiterpene biosynthesis. Comparison with ESTs derived from trichome collections of other plant species revealed expressed genes in common functional categories across different plant species. RT-PCR analysis confirmed the expression of selected unigenes and novel transcripts in A. annua glandular trichomes. The presence of contigs corresponding to enzymes for terpenoids and flavonoids biosynthesis suggests important metabolic activity in A. annua glandular trichomes. Our comprehensive survey of genes expressed in glandular trichome will facilitate new gene discovery and shed light on the regulatory mechanism of artemisinin metabolism and trichome function in A. annua.
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影响因子：
12.3
作者：
Huse SM;Huber JA;Morrison HG;Sogin ML;Welch DM
通讯作者：
Welch DM
DOI：
10.1073/pnas.092152999
发表时间：
2002-05-14
影响因子：
11.1
作者：
Bao, XM;Katz, S;Ohlrogge, J
通讯作者：
Ohlrogge, J
影响因子：
3.5
作者：
Lichtenthaler, HK;Schwender, J;Rohmer, M
通讯作者：
Rohmer, M
影响因子：
3.5
作者：
Matsushita, Y;Kang, W;Charlwood, BV
通讯作者：
Charlwood, BV
影响因子：
2.3
作者：
DUKE, SO;PAUL, RN
通讯作者：
PAUL, RN