Transcriptome Sequencing Analysis Reveals a Difference in Monoterpene Biosynthesis between Scented Lilium 'Siberia' and Unscented Lilium 'Novano'.
Transcriptome Sequencing Analysis Reveals a Difference in Monoterpene Biosynthesis between Scented Lilium 'Siberia' and Unscented Lilium 'Novano'.
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DOI：
10.3389/fpls.2017.01351
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发表时间：
2017
影响因子：
5.6
通讯作者：
Zhang K
中科院分区：
文献类型：
--
作者：
Hu Z;Tang B;Wu Q;Zheng J;Leng P;Zhang K
百合是世界著名的球茎香花，具有很高的观赏价值和经济价值，不同基因型百合的香气有显著差异。为了探索不同香味背后的机制，我们使用动态顶空技术在体内收集了气味强烈的百合“Sibeia”和气味微弱的百合“Novano”的花香。利用自动热解吸-气相色谱/质谱（ATD-GC/MS）对不同花期的香气进行了鉴定。我们利用RNA-Seq技术测定了开花期花瓣转录组，并分析了差异表达基因（DEGs），探讨了花香生物合成的分子机制。结果表明，与“Novano”百合相比，“西伯利亚”百合的花香释放量明显更高。此外，单萜烯在西伯利亚百合的花香中起主导作用；因此，我们认为单萜烯的释放量不同是两种百合基因型花香差异的主要原因。转录组测序分析结果显示，共生成~29.24 Gb的原始数据，组装成124,233个unigenes，其中35,749个unigenes被注释。通过比较这两种百合基因型的基因表达，鉴定出6496个deg。萜类主干生物合成途径基因的表达水平存在显著差异。1-脱氧-d -木酮糖5-磷酸合成酶（DXS）、1-脱氧-d -木酮糖-5-磷酸还原异构酶（DXR）、4-羟基-3-甲基-2-烯基二磷酸合成酶（HDS）、4-羟基-3-甲基-2-烯基二磷酸还原酶（HDR）、异戊二烯基二磷酸异构酶（IDI）和香叶二磷酸合成酶（GPS/GGPS）基因在西伯利亚百合中表达量均高于Novano百合；在西伯利亚百合的花被片中也有较高的表达量，这与单萜烯的释放量一致。我们证明了这些途径的高激活水平有助于西伯利亚百合的单萜生物合成，导致单萜的高积累和排放，这导致了两种百合基因型之间的香味差异。
Lilium is a world famous fragrant bulb flower with high ornamental and economic values, and significant differences in fragrance are found among different Lilium genotypes. In order to explore the mechanism underlying the different fragrances, the floral scents of Lilium ‘Sibeia’, with a strong fragrance, and Lilium ‘Novano’, with a very faint fragrance, were collected in vivo using a dynamic headspace technique. These scents were identified using automated thermal desorption—gas chromatography/mass spectrometry (ATD-GC/MS) at different flowering stages. We used RNA-Seq technique to determine the petal transcriptome at the full-bloom stage and analyzed differentially expressed genes (DEGs) to investigate the molecular mechanism of floral scent biosynthesis. The results showed that a significantly higher amount of Lilium ‘Siberia’ floral scent was released compared with Lilium ‘Novano’. Moreover, monoterpenes played a dominant role in the floral scent of Lilium ‘Siberia’; therefore, it is believed that the different emissions of monoterpenes mainly contributed to the difference in the floral scent between the two Lilium genotypes. Transcriptome sequencing analysis indicated that ~29.24 Gb of raw data were generated and assembled into 124,233 unigenes, of which 35,749 unigenes were annotated. Through a comparison of gene expression between these two Lilium genotypes, 6,496 DEGs were identified. The genes in the terpenoid backbone biosynthesis pathway showed significantly different expression levels. The gene expressions of 1-deoxy-D-xylulose 5-phosphate synthase (DXS), 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR), 4-hydroxy-3-methylbut-2-enyl diphosphate synthase (HDS), 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (HDR), isopentenyl diphosphate isomerase (IDI), and geranyl diphosphate synthase (GPS/GGPS), were upregulated in Lilium ‘Siberia’ compared to Lilium ‘Novano’, and two monoterpene synthase genes, ocimene synthase gene (OCS) and myrcene synthase gene (MYS), were also expressed at higher levels in the tepals of Lilium ‘Siberia’, which was consistent with the monoterpene release amounts. We demonstrated that the high activation levels of the pathways contributed to monoterpene biosynthesis in Lilium ‘Siberia’ resulting in high accumulations and emissions of monoterpenes, which led to the difference in fragrance between these two Lilium genotypes.
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DOI：
10.1073/pnas.96.23.13062
发表时间：
1999-11-09
影响因子：
11.1
作者：
Burke, CC;Wildung, MR;Croteau, R
通讯作者：
Croteau, R
影响因子：
7.2
作者：
Bouvier, F;Suire, C;Camara, B
通讯作者：
Camara, B
影响因子：
4.8
作者：
Estévez, JM;Cantero, A;León, P
通讯作者：
León, P
影响因子：
2.9
作者：
Hsieh, Wei-Yu;Hsieh, Ming-Hsiun
通讯作者：
Hsieh, Ming-Hsiun
影响因子：
6.5
作者：
Hu, Zenghui;Li, Tianjiao;Leng, Pingsheng
通讯作者：
Leng, Pingsheng